LOCUS       JK034146                 893 bp    mRNA    linear   EST 07-FEB-2014
DEFINITION  64 (LHA-2011) Bellamya aeruginosa normalized cDNA library Bellamya
            aeruginosa cDNA 5', mRNA sequence.
ACCESSION   JK034146
VERSION     JK034146.1
DBLINK      BioSample: SAMN00622379
KEYWORDS    EST.
SOURCE      Sinotaia aeruginosa
  ORGANISM  Sinotaia aeruginosa
            Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
            Caenogastropoda; Architaenioglossa; Viviparoidea; Viviparidae;
            Sinotaia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   An,L.H. and Zheng,B.H.
  TITLE     Construction and Characterization of Normalized cDNA Library from
            the Snail Bellamya aeruginosa Exposure to Copper
  JOURNAL   Unpublished
COMMENT     Contact: An L.H.
            State Environmental Protection Key Laboratory Of Estuarine And
            Coastal Research
            Chinese Research Academy For Environment Sciences
            8 Dayangfang BeiYuan Road, Chaoyang District, Beijing 100012, China
            Tel: +86-10-84913914
            Fax: +86-10-84913914
            Email: anlh@craes.org.cn
            PCR PRimers
            FORWARD: CTCGGGAAGCGCGCCATTGTGTTGGT
            BACKWARD: ATACGTCTCACTATAGGGCGAATTGGCC.
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Sinotaia aeruginosa"
                     /mol_type="mRNA"
                     /db_xref="taxon:2653872"
                     /tissue_type="gonads; digestive gland; peripheral tissue"
                     /clone_lib="SAMN00622379 Bellamya aeruginosa normalized
                     cDNA library"
                     /dev_stage="mature"
                     /note="Vector: Lambda TripEx2; 1.5 microliter RNA solution
                     (1-3 microgram of total RNA) was normalized using CDS and
                     SMART Oligo IV primer firstly, and then the reaction
                     solution was reversed transcription for synthesizing the
                     first-strand using MMLV reverse transcriptase (Promega,
                     USA). Subsequently, PCR reaction was operated and cDNA was
                     extracted using Promegas PCR clean-up system kit (Madison,
                     USA). And then the cDNA library was constructed using
                     SMART cDNA Library Construction Kit. After that, 6000
                     random clones were sequenced by the ABI3730xl. BLAST
                     homology searches in the nr and nt databases in GenBank
                     were performed for each of the unigenes under Evalues of
                     1e-5. The unigenes were named after the homologous
                     sequences in GenBank. The annotated unigenes were then
                     matched to specific processes or functions using COG and
                     GO tools."
BASE COUNT          255 a          200 c          217 g          221 t
ORIGIN      
        1 gttaattata ttgcaaacac tggcatgtga aggtgcaggt atatactaaa gttaacaagt
       61 ctgcggagaa gaggtgtacc tgttgctgta accactgttg gcgtcacaca acagacacaa
      121 cagatcagta gacatggtca acaggtggat gtggattttg atggttatca ttttccattg
      181 ctgcaagtat gtctgctctc gagctttaca ggaatcaatg attaacccag aagaaaacat
      241 cggcgtgaat gaatatttat cagaaggaga cattagaatt tcacggcgaa ggaatgcggt
      301 tcgagacttt tctcaaacgt gggttaaccg cactgtgcct tacaccgtgg agaaacttag
      361 tgatacagaa catcagcaag ttgtcatggc tatgcgagag ttatctgcca aatcctgcgt
      421 ccgttttgta ccgcgtacca ttcaaaggga ttatatcact atagtcaaac tcgatggttg
      481 cagcagttcc gtgggaaggc gtggtggaca gcaacaagtc atccttggag acggttgcta
      541 cgagaaaggc accattatgc acgagctaat gcacgcgctt ggattctggc acgagcacag
      601 ccgccctgat cgtgacgact tcatccagat tatctggagc aatattgcac aggagcacaa
      661 gtccaatttt gaaagatatg accaatcagc tatcgacacg ctcaacactg tttacgacta
      721 cggttccatc atgcattaca agaacgacac gtttgccatg gaccggagca agatcacaat
      781 taacgccacc ttaccgctgc ccccaggggt ggagatgggt cagcgggtca tgctatccga
      841 cacagatatt gtgaggctct cacggctgta cagatgtaac attactcatt gct
//