LOCUS       JK034132                 919 bp    mRNA    linear   EST 07-FEB-2014
DEFINITION  50 (LHA-2011) Bellamya aeruginosa normalized cDNA library Bellamya
            aeruginosa cDNA 5', mRNA sequence.
ACCESSION   JK034132
VERSION     JK034132.1
DBLINK      BioSample: SAMN00622379
KEYWORDS    EST.
SOURCE      Sinotaia aeruginosa
  ORGANISM  Sinotaia aeruginosa
            Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
            Caenogastropoda; Architaenioglossa; Viviparoidea; Viviparidae;
            Sinotaia.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   An,L.H. and Zheng,B.H.
  TITLE     Construction and Characterization of Normalized cDNA Library from
            the Snail Bellamya aeruginosa Exposure to Copper
  JOURNAL   Unpublished
COMMENT     Contact: An L.H.
            State Environmental Protection Key Laboratory Of Estuarine And
            Coastal Research
            Chinese Research Academy For Environment Sciences
            8 Dayangfang BeiYuan Road, Chaoyang District, Beijing 100012, China
            Tel: +86-10-84913914
            Fax: +86-10-84913914
            Email: anlh@craes.org.cn
            PCR PRimers
            FORWARD: CTCGGGAAGCGCGCCATTGTGTTGGT
            BACKWARD: ATACGTCTCACTATAGGGCGAATTGGCC.
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Sinotaia aeruginosa"
                     /mol_type="mRNA"
                     /db_xref="taxon:2653872"
                     /tissue_type="gonads; digestive gland; peripheral tissue"
                     /clone_lib="SAMN00622379 Bellamya aeruginosa normalized
                     cDNA library"
                     /dev_stage="mature"
                     /note="Vector: Lambda TripEx2; 1.5 microliter RNA solution
                     (1-3 microgram of total RNA) was normalized using CDS and
                     SMART Oligo IV primer firstly, and then the reaction
                     solution was reversed transcription for synthesizing the
                     first-strand using MMLV reverse transcriptase (Promega,
                     USA). Subsequently, PCR reaction was operated and cDNA was
                     extracted using Promegas PCR clean-up system kit (Madison,
                     USA). And then the cDNA library was constructed using
                     SMART cDNA Library Construction Kit. After that, 6000
                     random clones were sequenced by the ABI3730xl. BLAST
                     homology searches in the nr and nt databases in GenBank
                     were performed for each of the unigenes under Evalues of
                     1e-5. The unigenes were named after the homologous
                     sequences in GenBank. The annotated unigenes were then
                     matched to specific processes or functions using COG and
                     GO tools."
BASE COUNT          287 a          214 c          209 g          209 t
ORIGIN      
        1 ggtccgcacc gaaaagatgg cgtgtgcttt tctttgttag aaactgtcca tatttttgat
       61 attttatgga caacatatag ttgctttgct gattgcagaa gttgtgattc agtgcattaa
      121 gtagtatttc tgacaaccaa gatacttcag acatggctct tcccaacttt cgtgcccaaa
      181 catctccagt accacagaga gctcgcaata ctccaggtac acctcagaca tcagaaaagg
      241 ctgagaaatc aagtggaagc ccatttgttc agtcaccaca tggtcatcct ggctttaacc
      301 caaccaaatc ccgtgttaaa gtctcaggat catcagagag attgcccaaa ccgcccaaac
      361 ctcctgacaa gcccttgatg ccgtatatga gatacagcag aaagtttcaa agcatggtgc
      421 atacatatca gaaggtttgg gagcaggtga aggcccaaaa cccagatctc aagctgtggg
      481 agatcggcaa gatcataggg cagatgtggc gcgacctatc agaaacagaa aaacaggagt
      541 acacagagga gtatgagaca gagaaggcaa cttacaatga gtcaatgaag gtttaccaca
      601 actcaccagc ttatcaggct tgggtagctg ccaaaggtaa agctgaaagg gaaagagaag
      661 cagctgctgc tgtagaggaa gataagccac ctgctcggcc tgacagaacc acaacatcgt
      721 cccggcagtc acaaagtcaa aaacttgaga tgccacgaat cagcattcag ccagcagaag
      781 atgatgatga tccagatgat ggattttcag taaaacatat tgcccatgct cgctacattc
      841 gtaaccaccg cctgattaat gagattttta gtgacagcgt tgtacctgat gtccgcacag
      901 tcgtcactac tggacgcat
//