LOCUS       JK034111                 887 bp    mRNA    linear   EST 07-FEB-2014
DEFINITION  29 (LHA-2011) Bellamya aeruginosa normalized cDNA library Bellamya
            aeruginosa cDNA 5', mRNA sequence.
ACCESSION   JK034111
VERSION     JK034111.1
DBLINK      BioSample: SAMN00622379
KEYWORDS    EST.
SOURCE      Sinotaia aeruginosa
  ORGANISM  Sinotaia aeruginosa
            Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
            Caenogastropoda; Architaenioglossa; Viviparoidea; Viviparidae;
            Sinotaia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   An,L.H. and Zheng,B.H.
  TITLE     Construction and Characterization of Normalized cDNA Library from
            the Snail Bellamya aeruginosa Exposure to Copper
  JOURNAL   Unpublished
COMMENT     Contact: An L.H.
            State Environmental Protection Key Laboratory Of Estuarine And
            Coastal Research
            Chinese Research Academy For Environment Sciences
            8 Dayangfang BeiYuan Road, Chaoyang District, Beijing 100012, China
            Tel: +86-10-84913914
            Fax: +86-10-84913914
            Email: anlh@craes.org.cn
            PCR PRimers
            FORWARD: CTCGGGAAGCGCGCCATTGTGTTGGT
            BACKWARD: ATACGTCTCACTATAGGGCGAATTGGCC.
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Sinotaia aeruginosa"
                     /mol_type="mRNA"
                     /db_xref="taxon:2653872"
                     /tissue_type="gonads; digestive gland; peripheral tissue"
                     /clone_lib="SAMN00622379 Bellamya aeruginosa normalized
                     cDNA library"
                     /dev_stage="mature"
                     /note="Vector: Lambda TripEx2; 1.5 microliter RNA solution
                     (1-3 microgram of total RNA) was normalized using CDS and
                     SMART Oligo IV primer firstly, and then the reaction
                     solution was reversed transcription for synthesizing the
                     first-strand using MMLV reverse transcriptase (Promega,
                     USA). Subsequently, PCR reaction was operated and cDNA was
                     extracted using Promegas PCR clean-up system kit (Madison,
                     USA). And then the cDNA library was constructed using
                     SMART cDNA Library Construction Kit. After that, 6000
                     random clones were sequenced by the ABI3730xl. BLAST
                     homology searches in the nr and nt databases in GenBank
                     were performed for each of the unigenes under Evalues of
                     1e-5. The unigenes were named after the homologous
                     sequences in GenBank. The annotated unigenes were then
                     matched to specific processes or functions using COG and
                     GO tools."
BASE COUNT          233 a          215 c          239 g          200 t
ORIGIN      
        1 gggcatgcct gtgcataaca gctcacaagt acattaaagg tgtgccattt ctcactaact
       61 ggacagataa catccaactc cgtttctaaa gtgttacagt tctgtctgcg tcggacatat
      121 ttcagaggaa tgcccgcccg ctagataacc acaacagaat aatcaggagc tagcactggg
      181 caactcaaac tagattgaaa atatcagaag atggctgacg ggtataaact gatggtcaag
      241 cacgtcggac aaattgtaca agtcagttcc acgcgacaga aggccaaata tggccctgat
      301 atgaaaaatg ttgacatttt ggagggagag acgggaggct attctctcct cgtggacagg
      361 aacggcttgc tggctgacat tggtcctaca gtcgttttgg acgagaagta ccgtcaggca
      421 aaaatcgatc agattctgga cgcttgcgga aagtgtgttt tacctggact tgtagacgca
      481 catacacatg cagtttgggc cggagaccga gtgcatgagt ttacgatgaa gctggctgga
      541 gccacttaca tggagatcca cgaggcaggg ggtggcattt atttcaccgt gaaccacacc
      601 cgcagcgcca gcgatgacac actgttccac ttacttcgag tgcgtcttct gaagatgctg
      661 cgctccggca cgaccttggt ggaggtcaaa agtggctatg gccttgacct tgacaacgaa
      721 gtgaagatgc tacgcgtgat tgaacgtgcc cgacgagccg tacccattga catctcgtgc
      781 acgtactgcg gggctcacgc cgtgcccatt ggtcagactg cccaggaggc aacacaaaac
      841 gtgttacaag tgcaactgcc tcggattctg gagctgacgt cgtctgg
//