LOCUS BLYCBX 1424 bp mRNA linear PLN 27-APR-1993
DEFINITION H.vulgare (barley) carboxypeptidase I mRNA, 3' end.
ACCESSION J03897
VERSION J03897.1
KEYWORDS carboxypeptidase.
SOURCE Hordeum vulgare
ORGANISM Hordeum vulgare
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
REFERENCE 1 (bases 1 to 1424)
AUTHORS Doan,N.P. and Fincher,G.B.
TITLE The A- and B-chains of carboxypeptidase I from germinated barley
originate from a single precursor polypeptide
JOURNAL J. Biol. Chem. 263 (23), 11106-11110 (1988)
PUBMED 3403516
COMMENT Original source text: Barley (H.vulgare L.) scutellum, cDNA to
mRNA, clone lambda c-1.
Draft entry and computer-readable sequence for [1] kindly provided
by G.B.Fincher, 10-MAY-1988.
FEATURES Location/Qualifiers
source 1..1424
/organism="Hordeum vulgare"
/mol_type="mRNA"
/db_xref="taxon:4513"
mRNA <1..1424
/product="CBXI mRNA"
CDS <1..1239
/note="carboxypeptidase I precursor"
/codon_start=1
/protein_id="AAA32940.1"
/translation="GGPGCSSFDGFVYEHGPFNFESGGSVKSLPKLHLNPYAWSKVST
MIYLDSPAGVGLSYSKNVSDYETGDLKTATDSHTFLLKWFQLYPEFLSNPFYIAGESY
AGVYVPTLSHEVVKGIQGGAKPTINFKGYMVGNGVCDTIFDGNALVPFAHGMGLISDE
IYQQASTSCHGNYWNATDGKCDTAISKIESLISGLNIYDILEPCYHSRSIKEVNLQNS
KLPQSFKDLGTTNKPFPVRTRMLGRAWPLRAPVKAGRVPSWQEVASGVPCMSDEVATA
WLDNAAVRSAIHAQSVSAIGPWLLCTDKLYFVHDAGSMIAYHKNLTSQGYRAIIFSGD
HDMCVPFTGSEAWTKSLGYGVVDSWRPWITNGQVSGYTEGYEHGLTFATIKGAGHTVP
EYKPQEAFAFYSRWLAGSKL"
mat_peptide <1..627
/product="carboxypeptidase I A chain"
mat_peptide 793..1236
/product="carboxypeptidase I B chain"
BASE COUNT 389 a 329 c 347 g 359 t
ORIGIN 572 bp upstream of Sau3A site.
1 ggcggccccg gctgctccag cttcgacggc ttcgtctacg agcacgggcc attcaatttc
61 gagtcgggcg ggtcagttaa aagcctgcca aagctccatc tcaaccctta tgcctggtcc
121 aaggtgtcta ccatgatata cctggactcc cctgctggcg tggggctgtc atactcgaag
181 aatgtttcag attatgaaac gggagacctc aagaccgcga cggattcgca tactttcctt
241 ctcaagtggt tccaattgta ccctgagttc ctgagcaacc cgttctacat agctggggag
301 tcgtatgctg gggtttatgt ccctactctt tcgcacgaag tcgtcaaagg gatacaagga
361 ggagctaagc caactataaa cttcaagggc tacatggtcg ggaatggcgt ctgcgacact
421 atctttgatg gcaatgctct tgtgccattt gcacatggaa tgggtctaat atcagatgaa
481 atataccagc aagctagtac gtcgtgccac ggaaattact ggaatgccac tgacggtaaa
541 tgtgataccg ctatatcgaa aatcgaatcg ttgatcagcg gacttaacat ttatgacatt
601 cttgagccat gctaccacag cagaagcatc aaagaagtga acctgcagaa tagcaaattg
661 cctcaaagtt tcaaagatct tggtacaact aacaagccat ttcccgtaag aacgagaatg
721 ctggggcgtg cttggccttt gagagctcct gtaaaagccg gacgtgttcc gtcatggcag
781 gaagttgcca gtggtgttcc atgcatgagc gacgaagttg caacagcatg gcttgacaac
841 gctgccgtca gatctgcaat ccatgcccaa tcagtaagtg caattgggcc atggctctta
901 tgcacagata aattgtattt cgttcatgat gctggtagta tgatcgctta tcacaagaac
961 ctcacaagcc agggttatcg tgccatcata ttcagtggcg accacgacat gtgtgtgcca
1021 ttcaccggga gtgaagcatg gacaaaatct ttggggtatg gagttgttga ctcgtggcgg
1081 ccatggatca caaacggaca agtttctggg tacaccgaag gatatgaaca tggtctcact
1141 tttgctacta ttaagggtgc tggacacacc gttcctgagt acaaaccaca ggaagcattt
1201 gctttctaca gccgttggct cgctggttca aagttgtgaa gcttgtgccc agctacaaat
1261 aatggggaat atacttctgc atacagtcaa gaatgaacaa atagagactt gaagaagaaa
1321 gagatggcct acacgccata tagatagtca catacccaaa tatcaaaaat agcaattgga
1381 acactgtgcg ttgtattctt tcatcaaata tatttggtcg atgc
//