LOCUS       H07459                   258 bp    mRNA    linear   EST 08-MAY-2010
DEFINITION  mbn177 BNL1 Brassica napus cDNA 3' similar to OEE 1, mRNA sequence.
ACCESSION   H07459
VERSION     H07459.1
DBLINK      BioSample: SAMN00154732
KEYWORDS    EST.
SOURCE      Brassica napus (rape)
  ORGANISM  Brassica napus
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Brassiceae; Brassica.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Lee,C.M., Lee,Y.J., Lee,M.H., Nam,H.G., Cho,T.J., Hahn,T.R.,
            Cho,M.J. and Sohn,U.
  TITLE     Large-scale analysis of expressed genes from the leaf of oilseed
            rape (Brassica napus L.)
  JOURNAL   Plant Cell Rep. 17 (12), 930-936 (1998)
COMMENT     Contact: Uik Sohn
            Laboratory of Molecular Biology
            Kyungpook National University
            Dept. of Genetic Eng.,Kyungpook National Univ.,Taegu 702-701,Korea
            Tel: 0539505382
            Fax: 0539555327
            Email: usohn@bh.kyungpook.ac.kr
            EST is putatively  homologous to OEE 1 (GenPept, A21632)  in T.
            saestivum with 95 % identity
            Seq primer: M13 Forward.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Brassica napus"
                     /mol_type="mRNA"
                     /cultivar="Naehan"
                     /db_xref="taxon:3708"
                     /clone_lib="SAMN00154732 BNL1"
                     /lab_host="NM522"
                     /note="Vector: pT7T3D; Site_1: NotI; Site_2: EcoRI;
                     Poly(A)-mRNA was purified from the leaf of B.napus. cDNA
                     library was constructed from the mRNAs by oligo(dT)
                     priming and directionally cloned from the NotI site in the
                     vector pT7T3D (Pharmacia) to the EcoRI site."
BASE COUNT           52 a           69 c           62 g           75 t
ORIGIN      
        1 tctaactgct agcagagcgc tcttgctaac actcgtcggc gttgacggtg gaatgtcaac
       61 gtgaggctcg atcatgcaga actctcttgc cagcgctact gctaccggtg ctttgaaggt
      121 actgaacacg atctcagcat gcctatcgcg atcaatagtt ggactgactg gttggcagtt
      181 ccagattcac tttgacttcc atgtatgtct tgctctgtat ctcgtcataa gtgagtctct
      241 tcggagctcc ctctgcac
//