LOCUS H07459 258 bp mRNA linear EST 08-MAY-2010 DEFINITION mbn177 BNL1 Brassica napus cDNA 3' similar to OEE 1, mRNA sequence. ACCESSION H07459 VERSION H07459.1 DBLINK BioSample: SAMN00154732 KEYWORDS EST. SOURCE Brassica napus (rape) ORGANISM Brassica napus Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica. REFERENCE 1 (bases 1 to 258) AUTHORS Lee,C.M., Lee,Y.J., Lee,M.H., Nam,H.G., Cho,T.J., Hahn,T.R., Cho,M.J. and Sohn,U. TITLE Large-scale analysis of expressed genes from the leaf of oilseed rape (Brassica napus L.) JOURNAL Plant Cell Rep. 17 (12), 930-936 (1998) COMMENT Contact: Uik Sohn Laboratory of Molecular Biology Kyungpook National University Dept. of Genetic Eng.,Kyungpook National Univ.,Taegu 702-701,Korea Tel: 0539505382 Fax: 0539555327 Email: usohn@bh.kyungpook.ac.kr EST is putatively homologous to OEE 1 (GenPept, A21632) in T. saestivum with 95 % identity Seq primer: M13 Forward. FEATURES Location/Qualifiers source 1..258 /organism="Brassica napus" /mol_type="mRNA" /cultivar="Naehan" /db_xref="taxon:3708" /clone_lib="SAMN00154732 BNL1" /lab_host="NM522" /note="Vector: pT7T3D; Site_1: NotI; Site_2: EcoRI; Poly(A)-mRNA was purified from the leaf of B.napus. cDNA library was constructed from the mRNAs by oligo(dT) priming and directionally cloned from the NotI site in the vector pT7T3D (Pharmacia) to the EcoRI site." BASE COUNT 52 a 69 c 62 g 75 t ORIGIN 1 tctaactgct agcagagcgc tcttgctaac actcgtcggc gttgacggtg gaatgtcaac 61 gtgaggctcg atcatgcaga actctcttgc cagcgctact gctaccggtg ctttgaaggt 121 actgaacacg atctcagcat gcctatcgcg atcaatagtt ggactgactg gttggcagtt 181 ccagattcac tttgacttcc atgtatgtct tgctctgtat ctcgtcataa gtgagtctct 241 tcggagctcc ctctgcac //