LOCUS       H00032                   429 bp    mRNA    linear   EST 16-JUN-1995
DEFINITION  ph2g03u_19/1TV Outward Alu-primed hncDNA library Homo sapiens cDNA
            clone ph2g03u_19/1TV, mRNA sequence.
ACCESSION   H00032
VERSION     H00032.1
DBLINK      BioSample: SAMN00154861
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Vinogradova,T.V., Lebedev,Y.B., Kopantzev,E.P., Wagner,L.L.,
            Volik,S.V., Ermolaeva,O.D., Lavrentyeva,I., Monastyrskaya,G.S. and
            Sverdlov,E.D.
  TITLE     Outward Alu-primed hncDNA library
  JOURNAL   Unpublished
COMMENT     Contact: Sverdlov ED
            Structure and Function of Human Genes
            Shemyakin Institute of Bioorganic Chemistry
            16/10 Miklukho-Maklaya, Moscow, 117871, Russia
            Tel: 70953306529
            Fax: 70953306538
            Email: sverd@humgen.siobc.msk.su.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="ph2g03u_19/1TV"
                     /clone_lib="SAMN00154861 Outward Alu-primed hncDNA
                     library"
                     /note="Vector: pGEM-3Z; Site_1: EcoR1; Site_2: BamH1; The
                     library was constructed as described in [Obradovic, D.,
                     Borodin, A.M., Kopantzev, E.P., Wagner, L.L., Volik, S.V.,
                     Ermolaeva, O.D., Lebedev, Y.B., Monastyrskaya, G.S.,
                     Sverdlov, E.D. (1993) Bioorganicheskaya khimia, 20,
                     919-930]. This protocol is based on nested primer strategy
                     using Alu- specific primers (ALN3 and TC-65) that direct
                     the hncDNA synthesis outward of Alu repeats."
BASE COUNT          101 a           81 c           77 g          170 t
ORIGIN      
        1 taaaaaggtt tttttaaaag ccatccttgt agaggaggtc tactggctac acatcccctg
       61 ggttttcctt taagaatgtc tctatatttt acctttatcc ctaaaggcta ttttcactgg
      121 atatggactc ctgaattgac ggttttggtt tagaacttta atatgttata ccacttcctt
      181 ctggaatcca tcatttctgg tgagaaatcc acagtccccc aaatctttat tcccctatag
      241 gtgatgtatt attgtcctct ggctggtttc cagatttttc tttggcttta gtttcagcag
      301 ttagatgagg tgtgcacatc ttggggttcc tactgtttgg aatttgttca gcttcttgat
      361 ctgtaagtta atgtctttca acaaattgga aagtaatcaa ccaatgttcc taaaatttgt
      421 ttttgtttt
//