LOCUS H00032 429 bp mRNA linear EST 16-JUN-1995 DEFINITION ph2g03u_19/1TV Outward Alu-primed hncDNA library Homo sapiens cDNA clone ph2g03u_19/1TV, mRNA sequence. ACCESSION H00032 VERSION H00032.1 DBLINK BioSample: SAMN00154861 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 429) AUTHORS Vinogradova,T.V., Lebedev,Y.B., Kopantzev,E.P., Wagner,L.L., Volik,S.V., Ermolaeva,O.D., Lavrentyeva,I., Monastyrskaya,G.S. and Sverdlov,E.D. TITLE Outward Alu-primed hncDNA library JOURNAL Unpublished COMMENT Contact: Sverdlov ED Structure and Function of Human Genes Shemyakin Institute of Bioorganic Chemistry 16/10 Miklukho-Maklaya, Moscow, 117871, Russia Tel: 70953306529 Fax: 70953306538 Email: sverd@humgen.siobc.msk.su. FEATURES Location/Qualifiers source 1..429 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="ph2g03u_19/1TV" /clone_lib="SAMN00154861 Outward Alu-primed hncDNA library" /note="Vector: pGEM-3Z; Site_1: EcoR1; Site_2: BamH1; The library was constructed as described in [Obradovic, D., Borodin, A.M., Kopantzev, E.P., Wagner, L.L., Volik, S.V., Ermolaeva, O.D., Lebedev, Y.B., Monastyrskaya, G.S., Sverdlov, E.D. (1993) Bioorganicheskaya khimia, 20, 919-930]. This protocol is based on nested primer strategy using Alu- specific primers (ALN3 and TC-65) that direct the hncDNA synthesis outward of Alu repeats." BASE COUNT 101 a 81 c 77 g 170 t ORIGIN 1 taaaaaggtt tttttaaaag ccatccttgt agaggaggtc tactggctac acatcccctg 61 ggttttcctt taagaatgtc tctatatttt acctttatcc ctaaaggcta ttttcactgg 121 atatggactc ctgaattgac ggttttggtt tagaacttta atatgttata ccacttcctt 181 ctggaatcca tcatttctgg tgagaaatcc acagtccccc aaatctttat tcccctatag 241 gtgatgtatt attgtcctct ggctggtttc cagatttttc tttggcttta gtttcagcag 301 ttagatgagg tgtgcacatc ttggggttcc tactgtttgg aatttgttca gcttcttgat 361 ctgtaagtta atgtctttca acaaattgga aagtaatcaa ccaatgttcc taaaatttgt 421 ttttgtttt //