LOCUS       GO549392                 555 bp    mRNA    linear   EST 14-APR-2009
DEFINITION  Mdltb7524E13_e25440.g1 Apple_EST_Mdltb Malus domestica cDNA 5'
            similar to pir|F86447 F5D14.6 protein - Arabidopsis thaliana
            gb|AAF81326.1|AC007767_6 Strong similarity to an unknown protein
            F19D11.4 gi|7485759 from Arabidopsis thaliana BAC F19D11
            gb|AC005310. EST gb|AV535485 comes from this gene, mRNA sequence.
ACCESSION   GO549392
VERSION     GO549392.1
DBLINK      BioSample: SAMN00167510
KEYWORDS    EST.
SOURCE      Malus domestica (apple)
  ORGANISM  Malus domestica
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae;
            Maleae; Malus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Korban,S., Vodkin,L., Liu,L., Gasic,K., Gonzales,O., Hernandez,A.,
            Aldwinckle,H., Malnoy,M., Carroll,N., Goldsbrough,P., Orvis,K.,
            Clifton,S., Pape,D., Marra,M., Hillier,L., Martin,J., Wylie,T.,
            Dante,M., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Ronko,I.,
            Tsagareishvili,R., Kennedy,S., Waterston,R. and Wilson,R.
  TITLE     Apple Functional Genomics grant - NSF 0321702
  JOURNAL   Unpublished
COMMENT     Contact: Schuyler S. Korban
            Apple Functional Genomics grant - NSF 0321702
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material provided by M.Wisniewski/C.Bassett/ J.
            Norelli/T.Artlip Library constructed by K. Gasic/S. Korban Library
            sequenced by Washington University Genome Sequencing Center
            original QR value of 815 was extended to value 816 (,)
            This trace has been recalled with phred
            original value before phred recall for SR was 816
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -40UP.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Malus domestica"
                     /mol_type="mRNA"
                     /cultivar="Royal Gala"
                     /db_xref="taxon:3750"
                     /tissue_type="bark tissue"
                     /clone_lib="SAMN00167510 Apple_EST_Mdltb"
                     /dev_stage="plants subjected to 5 degrees C. for 24hrs"
                     /lab_host="DH10B cells"
                     /note="Vector: pBluescript II SK (+); Site_1: Not I;
                     Site_2: EcoRI; Total RNA was extracted separately from
                     bark tissue, using the method described by Gasic et
                     al.(2004). Poly(A)+mRNA was isolated twice from total RNA,
                     using the Oligotex Direct mRNA kit(Qiagen). mRNA was
                     reverse transcribed into double stranded cDNA using a
                     modified oligo18(dT) primer with an identifying tag
                     sequence. [Tag identification when sequencing from 5'end:
                     insert 18(A) TGCGAG]Double stranded cDNAs were size
                     selected (more then 500bp), adaptored with EcoRI adapters
                     at both ends and then digested with NotI. The cDNAs were
                     then directionally cloned into EcoRI-NotI digested pBSII
                     SK(+) phagemid vector(Stratagene). The total number of
                     white colony forming units (cfu) was 6.0x10e6 cfu. The
                     background of empty clones was less than 5%. Inserts
                     ranged from 0.8 kb to 12kb, as determined by PCR."
BASE COUNT          163 a          113 c           93 g          186 t
ORIGIN      
        1 gctcgcattt tttttttttt tttttaaaaa gtaaaaggcc aatagtatat tcaatttttg
       61 ccaacattgc aattctcagt attgtatttt gatttcatat cagatacaac tgcatagtag
      121 tgacattcga gagcttgccc attcgggttt gacacaacaa tagaacatta tcccactaag
      181 tggagtcggc tatagaaatc ttagaatgac aaccaaattg ctttgccttc tcatatattg
      241 actcatttgc caaacagaca gaagaaaacc caaaatgttt gtaatcatgt accaaagcag
      301 caatccagga gccctcatgc tagttaacat catacatatg caagaacgca ttatccttcc
      361 tggctgaaaa tcattttggt ggtgccatat tccatttatt tgatgcttcc ttgtgcttct
      421 tgtcttgata tagggataac gcgatggcgc ggcccttcga acatccaatt atcttcttca
      481 agggatttaa ccaccttgtt aagtgcatcc ataagatcgg attttataac aagtaatgct
      541 tcttgtttgc gatct
//