LOCUS       GO546549                 631 bp    mRNA    linear   EST 14-APR-2009
DEFINITION  Mdfb8004A12_e3507.b1 Apple_EST_Mdfb Malus domestica cDNA 3' similar
            to gb|AAF97824.1|AC034107_7 Strong similarity to EF-1-alpha-related
            GTP-binding protein (SUP1) from Nicotiana tabacum gb|L38828 and is
            a member of the elongation factor Tu PF|00009 family. ESTs
            gb|W43190, gb|W43332, gb|AI995372, gb|AV563399, gb|AV549134, mRNA
            sequence.
ACCESSION   GO546549
VERSION     GO546549.1
DBLINK      BioSample: SAMN00176549
KEYWORDS    EST.
SOURCE      Malus domestica (apple)
  ORGANISM  Malus domestica
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae;
            Maleae; Malus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Korban,S., Vodkin,L., Liu,L., Gasic,K., Gonzales,O., Hernandez,A.,
            Aldwinckle,H., Malnoy,M., Carroll,N., Goldsbrough,P., Orvis,K.,
            Clifton,S., Pape,D., Marra,M., Hillier,L., Martin,J., Wylie,T.,
            Dante,M., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Ronko,I.,
            Tsagareishvili,R., Kennedy,S., Waterston,R. and Wilson,R.
  TITLE     Apple Functional Genomics grant - NSF 0321702
  JOURNAL   Unpublished
COMMENT     Contact: Schuyler S. Korban
            Apple Functional Genomics grant - NSF 0321702
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material provided by S. Korban/H. Aldwinckle/ M. Malnoy
            Library constructed by D.O.Gonzalez/L.Vodkin Library sequenced by
            Washington University Genome Sequencing Center
            original QR value of 447 was extended to value 447 (,)
            This trace has been recalled with phred
            original value before phred recall for SR was 447
            Seq primer: -40UP.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Malus domestica"
                     /mol_type="mRNA"
                     /cultivar="red delicious"
                     /db_xref="taxon:3750"
                     /tissue_type="Leaves challenged with fire blight (Erwinia
                     amylovora)"
                     /clone_lib="SAMN00176549 Apple_EST_Mdfb"
                     /lab_host="DH10B ampicillin resistant"
                     /note="Vector: pBluescript II SK (+); Site_1: XhoI;
                     Site_2: EcoRI; Total RNA was extracted from freeze dried
                     leaf tissue, using the method described by Wang and Vodkin
                     (Plant Molecular Biology Reporter 12:132-145, 1994).
                     Poly(A)+ mRNA was isolated from total RNA using the
                     PolyATtract mRNA Isolation system III (Promega). The
                     library was prepared using the Stratagene pBluescript II
                     XR cDNA library construction kit. Complementary DNA was
                     synthesized from mRNA using a Poly (dT) sequence with a
                     XhoI restriction site. EcoRI adapters were ligated to the
                     blunt ended cDNA fragments followed by restriction with
                     XhoI. The cDNA insert is protected from XhoI digestion via
                     methylation during first strand cDNA synthesis. The cDNA
                     fragments were directionally cloned into the EcoRI-XhoI
                     restriction site of the pBluescript vector. The ligated
                     cDNA fragments were transformed into E. coli ElectroMax
                     DH10B host cells. Transformation efficiency: 1.30E+05
                     Average insert Size by PCR: 1420 bp"
BASE COUNT          172 a          102 c          124 g          233 t
ORIGIN      
        1 tctttcaagg tcaaggtatt gtttgaaaat ggattttcgg tcattggccc ctatggaaat
       61 tatacggagg gggaatgatg agggggggat cattggctcc ctaaaaattt attaaactgt
      121 caaagtaacc ccaatttggt gatttattgt taaatgtatt ctctttaatt ttctttaatc
      181 ccctgaattc ctttctgtag aatgcctatc ataaacaaat tcaaggactt ggaaacagtt
      241 gttatgggca aagtgaaatc tgggtccgtg cgtgaaggga attcattgtt tgtcttgcca
      301 aataaggccc cattgaaacc tgttgctata tactttgata aaaatagggg gaaccgtgcg
      361 gccccaggtg aaaatctacg ggttaaatta tcaggcattg ataataagaa catattgccg
      421 ggctttgttg tgtcaagtgt tggtaagttc tatttcacgg cgtttcactg aattctttgt
      481 ccattcttaa aagttttcaa gtattttatt ccttaaaagt aaaaagttgc attgctgctg
      541 catgttcgtt ttttcccctt taaagtttga accatttatc tttatttgtt tacagaacct
      601 gtcattattt attttatttc tttatttatc a
//