LOCUS       GO531225                 655 bp    mRNA    linear   EST 14-APR-2009
DEFINITION  Mdltb7524E14.g1 Apple_EST_Mdltb Malus domestica cDNA 5' similar to
            pir|F86447 F5D14.6 protein - Arabidopsis thaliana
            gb|AAF81326.1|AC007767_6 Strong similarity to an unknown protein
            F19D11.4 gi|7485759 from Arabidopsis thaliana BAC F19D11
            gb|AC005310. EST gb|AV535485 comes from this gene, mRNA sequence.
ACCESSION   GO531225
VERSION     GO531225.1
DBLINK      BioSample: SAMN00167510
KEYWORDS    EST.
SOURCE      Malus domestica (apple)
  ORGANISM  Malus domestica
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae;
            Maleae; Malus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Korban,S., Vodkin,L., Liu,L., Gasic,K., Gonzales,O., Hernandez,A.,
            Aldwinckle,H., Malnoy,M., Carroll,N., Goldsbrough,P., Orvis,K.,
            Clifton,S., Pape,D., Marra,M., Hillier,L., Martin,J., Wylie,T.,
            Dante,M., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Ronko,I.,
            Tsagareishvili,R., Kennedy,S., Waterston,R. and Wilson,R.
  TITLE     Apple Functional Genomics grant - NSF 0321702
  JOURNAL   Unpublished
COMMENT     Contact: Schuyler S. Korban
            Apple Functional Genomics grant - NSF 0321702
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material provided by M.Wisniewski/C.Bassett/ J.
            Norelli/T.Artlip Library constructed by K. Gasic/S. Korban Library
            sequenced by Washington University Genome Sequencing Center
            original QR value of 805 was extended to value 805 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 91
            original value before phred recall for SR was 812
            Seq primer: -40UP.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Malus domestica"
                     /mol_type="mRNA"
                     /cultivar="Royal Gala"
                     /db_xref="taxon:3750"
                     /tissue_type="bark tissue"
                     /clone_lib="SAMN00167510 Apple_EST_Mdltb"
                     /dev_stage="plants subjected to 5 degrees C. for 24hrs"
                     /lab_host="DH10B cells"
                     /note="Vector: pBluescript II SK (+); Site_1: Not I;
                     Site_2: EcoRI; Total RNA was extracted separately from
                     bark tissue, using the method described by Gasic et
                     al.(2004). Poly(A)+mRNA was isolated twice from total RNA,
                     using the Oligotex Direct mRNA kit(Qiagen). mRNA was
                     reverse transcribed into double stranded cDNA using a
                     modified oligo18(dT) primer with an identifying tag
                     sequence. [Tag identification when sequencing from 5'end:
                     insert 18(A) TGCGAG]Double stranded cDNAs were size
                     selected (more then 500bp), adaptored with EcoRI adapters
                     at both ends and then digested with NotI. The cDNAs were
                     then directionally cloned into EcoRI-NotI digested pBSII
                     SK(+) phagemid vector(Stratagene). The total number of
                     white colony forming units (cfu) was 6.0x10e6 cfu. The
                     background of empty clones was less than 5%. Inserts
                     ranged from 0.8 kb to 12kb, as determined by PCR."
BASE COUNT          188 a          144 c          140 g          183 t
ORIGIN      
        1 cgaattccgt tgctgtcgct tccttgtgcg acagtgagaa tgagtaattc gaattcatcg
       61 ccggcacatt cgtcgatatc gacgacagcg atagccggag aaagagaacg aagcaattcc
      121 aatcctgccg ccgccgccgc tctctccgac gacctcttct tcccctccga cctcatttcc
      181 attcaagatc gcaaacaaga agcattactt gttataaaat ccgatcttat ggatgcactt
      241 aacaaggtgg ttaaatccct tgaagaagat aattggatgt tcgaagggcc gcgccatcgc
      301 gttatcccta tatcaagaca agaagcacaa ggaagcatca aataaatgga atatggcacc
      361 accaaaatga ttttcagcca ggaaggataa tgcgttcttg catatgtatg atgttaacta
      421 gcatgagggc tcctggattg ctgctttggt acatgattac aaacattttg ggttttcttc
      481 tgtctgtttg gcaaatgagt caatatatga gaaggcaaag caatttggtt gtcattctaa
      541 gatttctata gccgactcca cttagtggga taatgttcta ttgttgtgtc aaacccgaat
      601 gggcaagctc tcgaatgtca ctactatgca gttgtatctg atatgaaatc aaaat
//