LOCUS GO525315 579 bp mRNA linear EST 14-APR-2009 DEFINITION Mdfrg3152E04.g1 Apple_EST_Mdfrg Malus domestica cDNA 5' similar to pir|F86447 F5D14.6 protein - Arabidopsis thaliana gb|AAF81326.1|AC007767_6 Strong similarity to an unknown protein F19D11.4 gi|7485759 from Arabidopsis thaliana BAC F19D11 gb|AC005310. EST gb|AV535485 comes from this gene, mRNA sequence. ACCESSION GO525315 VERSION GO525315.1 DBLINK BioSample: SAMN00149828 KEYWORDS EST. SOURCE Malus domestica (apple) ORGANISM Malus domestica Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus. REFERENCE 1 (bases 1 to 579) AUTHORS Korban,S., Vodkin,L., Liu,L., Gasic,K., Gonzales,O., Hernandez,A., Aldwinckle,H., Malnoy,M., Carroll,N., Goldsbrough,P., Orvis,K., Clifton,S., Pape,D., Marra,M., Hillier,L., Martin,J., Wylie,T., Dante,M., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Ronko,I., Tsagareishvili,R., Kennedy,S., Waterston,R. and Wilson,R. TITLE Apple Functional Genomics grant - NSF 0321702 JOURNAL Unpublished COMMENT Contact: Schuyler S. Korban Apple Functional Genomics grant - NSF 0321702 Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by S.S. Korban/H.S. Aldwinckle/M.Malnoy Library constructed by D.O.Gonzalez/L.Vodkin Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 110 original value before phred recall for SR was 833 Seq primer: -40RP. FEATURES Location/Qualifiers source 1..579 /organism="Malus domestica" /mol_type="mRNA" /cultivar="Granny" /db_xref="taxon:3750" /tissue_type="Fruit tissue" /clone_lib="SAMN00149828 Apple_EST_Mdfrg" /dev_stage="35 days after pollination" /lab_host="DH10B cells" /note="Vector: pBluescript II SK (+); Site_1: XhoI; Site_2: EcoRI; Total RNA was extracted from freeze dried fruit tissue, using the method described by Wang and Vodkin(Plant Molecular Biology Reporter 12:132-145, 1994). Poly(A)+mRNA was isolated from total RNA using the PolyATtract mRNA Isolation system III(Promega). The library was prepared using the Stratagene pBluescript II XR cDNA library construction kit. Complementary DNA was synthesized from mRNA using a Poly(dT)sequence with a Xho-I restriction site. EcoRI adapters were ligated to the blunt ended cDNA fragments followed by restriction with XhoI. The cDNA insert is protected from XhoI digestion via methylation during first strand cDNA synthesis. The cDNA fragments were directionally cloned into the EcoRI-XhoI restriction site of the pBluescript vector. The ligated cDNA fragments were transformed into E.coli ElectroMax DH10B host cells." BASE COUNT 163 a 134 c 123 g 159 t ORIGIN 1 cacgaggtac atttccaagt ctcttccttg tgcgacagtg agaatgagta attcgaattc 61 atcgccggca cattcgtcga tatcgacgac agcgatagcc ggagaaagag aacgaagcaa 121 ttccaatcct gccgccgccg ccgctctctc cgacgacctc ttcttcccct ccgacctcat 181 ttccattcaa gatcgcaaac acgaagcatt acttgttata aaatccgatc ttatggatgc 241 acttaacaag gtggttaaat cccttgaaga agataattgg atgttcgaag ggccgcgcca 301 tcgcgttatc cctatatcaa gacaaggcgg ttttctccag aagcacaagg aagcatcaaa 361 taaatggaat atggcaccac caaaatgatt ttcagccagg aaggataatg cgttcttgca 421 tatgtatgat gttaactagc atgagggctc ctggattgct gctttggtac gtgattacaa 481 acattttggg ttttcttctg tctgtttggt aaatgagtca atttatgaga aggcaaagca 541 atttggttgt cattctaaca tttatatagc cgactccac //