LOCUS       GO525315                 579 bp    mRNA    linear   EST 14-APR-2009
DEFINITION  Mdfrg3152E04.g1 Apple_EST_Mdfrg Malus domestica cDNA 5' similar to
            pir|F86447 F5D14.6 protein - Arabidopsis thaliana
            gb|AAF81326.1|AC007767_6 Strong similarity to an unknown protein
            F19D11.4 gi|7485759 from Arabidopsis thaliana BAC F19D11
            gb|AC005310. EST gb|AV535485 comes from this gene, mRNA sequence.
ACCESSION   GO525315
VERSION     GO525315.1
DBLINK      BioSample: SAMN00149828
KEYWORDS    EST.
SOURCE      Malus domestica (apple)
  ORGANISM  Malus domestica
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae;
            Maleae; Malus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Korban,S., Vodkin,L., Liu,L., Gasic,K., Gonzales,O., Hernandez,A.,
            Aldwinckle,H., Malnoy,M., Carroll,N., Goldsbrough,P., Orvis,K.,
            Clifton,S., Pape,D., Marra,M., Hillier,L., Martin,J., Wylie,T.,
            Dante,M., Theising,B., Bowers,Y., Gibbons,M., Ritter,E., Ronko,I.,
            Tsagareishvili,R., Kennedy,S., Waterston,R. and Wilson,R.
  TITLE     Apple Functional Genomics grant - NSF 0321702
  JOURNAL   Unpublished
COMMENT     Contact: Schuyler S. Korban
            Apple Functional Genomics grant - NSF 0321702
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material provided by S.S. Korban/H.S. Aldwinckle/M.Malnoy
            Library constructed by D.O.Gonzalez/L.Vodkin Library sequenced by
            Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 110
            original value before phred recall for SR was 833
            Seq primer: -40RP.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Malus domestica"
                     /mol_type="mRNA"
                     /cultivar="Granny"
                     /db_xref="taxon:3750"
                     /tissue_type="Fruit tissue"
                     /clone_lib="SAMN00149828 Apple_EST_Mdfrg"
                     /dev_stage="35 days after pollination"
                     /lab_host="DH10B cells"
                     /note="Vector: pBluescript II SK (+); Site_1: XhoI;
                     Site_2: EcoRI; Total RNA was extracted from freeze dried
                     fruit tissue, using the method described by Wang and
                     Vodkin(Plant Molecular Biology Reporter 12:132-145, 1994).
                     Poly(A)+mRNA was isolated from total RNA using the
                     PolyATtract mRNA Isolation system III(Promega). The
                     library was prepared using the Stratagene pBluescript II
                     XR cDNA library construction kit. Complementary DNA was
                     synthesized from mRNA using a Poly(dT)sequence with a
                     Xho-I restriction site. EcoRI adapters were ligated to the
                     blunt ended cDNA fragments followed by restriction with
                     XhoI. The cDNA insert is protected from XhoI digestion via
                     methylation during first strand cDNA synthesis. The cDNA
                     fragments were directionally cloned into the EcoRI-XhoI
                     restriction site of the pBluescript vector. The ligated
                     cDNA fragments were transformed into E.coli ElectroMax
                     DH10B host cells."
BASE COUNT          163 a          134 c          123 g          159 t
ORIGIN      
        1 cacgaggtac atttccaagt ctcttccttg tgcgacagtg agaatgagta attcgaattc
       61 atcgccggca cattcgtcga tatcgacgac agcgatagcc ggagaaagag aacgaagcaa
      121 ttccaatcct gccgccgccg ccgctctctc cgacgacctc ttcttcccct ccgacctcat
      181 ttccattcaa gatcgcaaac acgaagcatt acttgttata aaatccgatc ttatggatgc
      241 acttaacaag gtggttaaat cccttgaaga agataattgg atgttcgaag ggccgcgcca
      301 tcgcgttatc cctatatcaa gacaaggcgg ttttctccag aagcacaagg aagcatcaaa
      361 taaatggaat atggcaccac caaaatgatt ttcagccagg aaggataatg cgttcttgca
      421 tatgtatgat gttaactagc atgagggctc ctggattgct gctttggtac gtgattacaa
      481 acattttggg ttttcttctg tctgtttggt aaatgagtca atttatgaga aggcaaagca
      541 atttggttgt cattctaaca tttatatagc cgactccac
//