LOCUS G10777 282 bp DNA linear STS 19-AUG-1999 DEFINITION bnl8.45 R maize DGrant3 Zea mays STS genomic clone bnl8.45, sequence tagged site. ACCESSION G10777 VERSION G10777.1 KEYWORDS STS. SOURCE Zea mays ORGANISM Zea mays Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. REFERENCE 1 (bases 1 to 282) AUTHORS Grant,D. and Helentjaris,T. TITLE Zea mays RFLP probe JOURNAL Unpublished COMMENT Contact: David Grant, Tim Helentjaris Pioneer Hi-Bred Int., Inc. 7300 NW 62nd Ave., PO Box 1004, Johnston, IA 50131-1004, USA Email: dgrant@mendel.agron.iastate.edu, helentjartg@phibred.com Primer A: None Primer B: None STS size: 282 A single sequencing run was made using the clone and the universal Reverse sequencing primer. FEATURES Location/Qualifiers source 1..282 /organism="Zea mays" /mol_type="genomic DNA" /strain="inbred B37" /db_xref="taxon:4577" /clone="bnl8.45" /clone_lib="maize DGrant3" /note="Vector: pUC8; V-type: Plasmid; PstI-generated genomic DNA fragment from maize inbred B37 cloned into the PstI site of pUC8. See Burr et al. (1988) Genetics 11" STS <1..>282 BASE COUNT 54 a 70 c 81 g 77 t ORIGIN 1 ctagagccgg ggacaacgtc gccgttggtt tgcacggggt ggaccctggc cacatcgcgg 61 caggcggagt tctctgccat ccagatttcc cggtgcgcgt ggcgtgtcac ctggagctga 121 aaattctggt tctggaaatc accgtgccaa tactggttgg ccttgaggtc agtgggaaac 181 agcacgacga atagcattgt tcttgttcgt ctggtgcgat cttctttatc tgtttgtttc 241 catgagaaac cattatttcc agtttgagct gcacatacgg ca //