LOCUS       G05162                   251 bp    DNA     linear   STS 28-SEP-1998
DEFINITION  2P-PCR-H Hordeum Wang&Wei Hordeum vulgare STS genomic, sequence
            tagged site.
ACCESSION   G05162
VERSION     G05162.1
KEYWORDS    STS.
SOURCE      Hordeum vulgare
  ORGANISM  Hordeum vulgare
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Wang,R.R. and Wei,J.Z.
  TITLE     Variations of two repetitive DNA sequences in several Triticeae
            genomes revealed by polymerase chain reaction and sequencing
  JOURNAL   Genome 38 (6), 1221-1229 (1995)
   PUBMED   8654916
COMMENT     
            Contact: Richard Wang
            Forage and Range Research Laboratory
            USDA-ARS, Utah State University
            695 N 1100 E, Logan, UT 84322-6300, USA
            Email: rrcwang@cc.usu.edu
            Primer A: ACAATCTGAAAATCTGGACA
            Primer B: TCATATTGAGACTCCTATAA
            STS size: 251
            PCR Profile:
                    Presoak:            0 degrees C for 0.00 minute(s)
                    Denaturation:    93 degrees C for 1.00 minute(s)
                    Annealing:         52 degrees C for 1.00 minute(s)
                    Polymerization: 71 degrees C for 2.00 minute(s)
                    PCR Cycles:      28
                    Thermal Cycler: Perkin Elmer 9600
            Protocol:
                    Template:       40 ng
                    Primer:         each 3 uM
                    dNTPs:          each 200 uM
                    Taq Polymerase: 0.04 units/ul
                    Total Vol:      25 ul
            
            Buffer:
                    MgCl2:           2.5 mM
                    KCl:              50 mM
                    Tris-HCl:         10 mM
                    pH:                 8.3.
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Hordeum vulgare"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:4513"
                     /clone_lib="Hordeum Wang&Wei"
                     /note="V-type: Unknown"
     STS             1..>251
     primer_bind     1..20
BASE COUNT           81 a           37 c           55 g           78 t
ORIGIN      
        1 acaatctgaa aatctggaca gaatttgcaa atggatgctg tgctcaaact ttgaactatg
       61 gaacttgggt tctggtggaa atggggaaga tatggatagg agaagctcca caatttattt
      121 gggatttttc ctggctacca aaatggtagt tcctttagaa agtgcccaaa atgcaatatt
      181 ggcattaaat aggaaaaggc aatttcccct atttaattat ggccaatatt tttattggag
      241 tctcaatatg a
//