LOCUS       FX175685                 792 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Pandanus boninensis mRNA, contig: Pb_c13935.
ACCESSION   FX175685
VERSION     FX175685.1
DBLINK      BioProject:PRJDA67377
            Sequence Read Archive:DRR001102
            BioSample:SAMD00011606
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Pandanus boninensis
  ORGANISM  Pandanus boninensis
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; Liliopsida; Pandanales; Pandanaceae;
            Pandanus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K. and Yoshimaru,H.
  TITLE     Development of EST-SSR markers from cDNA library of Pandanus
            boninensis Warburg (Pandanaceae)
  JOURNAL   Unpublished (2011)
COMMENT     Several-year-old seedlings of Pandanus boninensis naturally grown
            in Bonin Island were used for RNA extraction. About 3 g of inner
            bark was cut and frozen in liquid nitrogen, ground using mortar
            and pestle. Total RNA was extracted by the CTAB method (Le Provost
            et al., 2007), and contaminated DNA was removed by SV total RNA
            isolation system (Promega, Madison, WI). The cDNA library
            construction and next generation sequencing by Roche 454 GS FLX
            sequencer with titanium chemistry were carried out by the Hokkaido
            System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and
            adapter sequences from the cDNA sequences, the Program cross_match
            (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR
            SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..792
                     /db_xref="taxon:1035881"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Pb_c13935"
                     /organism="Pandanus boninensis"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          352 a          108 c          135 g          192 t
ORIGIN      
        1 ctagaactag ggaaaggaat acgagggata tgaatcaagt tattaaaact aaagatcata
       61 gaattttagt gaaagacatg gacatcaaag agagataaaa atgttatttt aaaaggttat
      121 taaataagaa ttcgactata aaagtagatt tagaagatac ctatatgatg catgcaacct
      181 aagcataaaa ttgtttctac tcttgtaatg caacttttat tagattcaag taagggcgga
      241 ttttagatta ataaaaccta cactagaaag caataaagga attaaagagg ggaagatgga
      301 aagaaatcac ctgaaaagta agcaattttt ttttaacaaa cacaatcaaa gacaatctct
      361 atcttctacc tggctgcaac aagaagcaaa caaaawaaag agactagtta gtactaatga
      421 atgyaaaacg agagcaaaga taatcaaacc caaaacttgc ttccccasgc aacggcrcca
      481 aaaattaata cgagtttrca aggtgtcgtt aagcaatgca aatgtatcaa attagtttcg
      541 acttgcaaca aaagtaaaaa cgtagtacaa tagcaagcac aggtcgaatt ccacggggaa
      601 gaaaggcaaa ggtttaaaaa ctttgatcaa actaatcaaa tgctaaaaac aaagaataat
      661 gaatttacta tttactaaaa taaagcttat aaataaatct taaataaata agaaggatca
      721 aacaagatga caatgcaaat ctaaaaataa agtgaaagag aaagatggga tgggcatgta
      781 cctttagaac tt
//