LOCUS       FX174296                 573 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Pandanus boninensis mRNA, contig: Pb_c12546.
VERSION     FX174296.1
DBLINK      BioProject:PRJDA67377
            Sequence Read Archive:DRR001102
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Pandanus boninensis
  ORGANISM  Pandanus boninensis
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Pandanales; Pandanaceae;
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K. and Yoshimaru,H.
  TITLE     Development of EST-SSR markers from cDNA library of Pandanus
            boninensis Warburg (Pandanaceae)
  JOURNAL   Unpublished (2011)
COMMENT     Several-year-old seedlings of Pandanus boninensis naturally grown
            in Bonin Island were used for RNA extraction. About 3 g of inner
            bark was cut and frozen in liquid nitrogen, ground using mortar
            and pestle. Total RNA was extracted by the CTAB method (Le Provost
            et al., 2007), and contaminated DNA was removed by SV total RNA
            isolation system (Promega, Madison, WI). The cDNA library
            construction and next generation sequencing by Roche 454 GS FLX
            sequencer with titanium chemistry were carried out by the Hokkaido
            System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and
            adapter sequences from the cDNA sequences, the Program cross_match
            ( and TIGR
            SeqClean sequence trimming pipeline
            ( was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..573
                     /dev_stage="several year old seedlings"
                     /note="contig: Pb_c12546"
                     /organism="Pandanus boninensis"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          205 a          123 c          112 g          128 t
        1 gaatgtggtt tgagtgttgc tagtgtgatt agaccaccaa tgctaagtga tcataactag
       61 taaaggccac gaaagcaaag cacataaaag atttaatatt gaaagcacaa atgtaactaa
      121 ggccctagaa acacaaacac agaaaaagtg aaaatcttct actttgtata gttgtykayw
      181 agatayctgt agttacaatt gtttgctgaa gcacaaccct tttcggtgca gcaacattgt
      241 acggaaagaa ctgtgatgaa agtgccagag caagcacact cacctgcact aagaaatgca
      301 caatggatgc ccttgccaat cgacgcccaa gaacaggtaa tagaggaccc atacaccacc
      361 cagtccacca ttccaattag tgctgccact atactatcag gaattaaata tccataaggc
      421 tgagggagag aacccatcat acccatcttc tcaatcacga actgaatcat aaacccacca
      481 atgtagacaa gataggctac acatgggatt agagggataa tgtatcctgc caatgacctg
      541 aaagactgat ggccaaaaag cttgcttgtt aga