LOCUS FX173773 654 bp mRNA linear TSA 23-MAY-2012 DEFINITION TSA: Pandanus boninensis mRNA, contig: Pb_c12023. ACCESSION FX173773 VERSION FX173773.1 DBLINK BioProject:PRJDA67377 Sequence Read Archive:DRR001102 BioSample:SAMD00011606 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Pandanus boninensis ORGANISM Pandanus boninensis Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Pandanales; Pandanaceae; Pandanus. REFERENCE 1 (bases 1 to 654) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Setsuko,S., Uchiyama,K., Sugai,K. and Yoshimaru,H. TITLE Development of EST-SSR markers from cDNA library of Pandanus boninensis Warburg (Pandanaceae) JOURNAL Unpublished (2011) COMMENT Several-year-old seedlings of Pandanus boninensis naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark was cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..654 /db_xref="taxon:1035881" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Pb_c12023" /organism="Pandanus boninensis" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 205 a 119 c 128 g 202 t ORIGIN 1 gcatttgtcc ctccaacaca gtttgaggct tcatgataat tgaaattttg tctactgtgt 61 tcataagcag tcatgcctta attatttcat caactgcata cttcagtatc gtgcagattt 121 tccaagcagc ttaaaggggc aagcagtttt tctagatata gcgccagact tttgtcatgt 181 ggcagaagac atactgcata agcaaattca actagttaga aatagtttga aagacgctgt 241 agatggtgcc aatgggtttc agaataccca tcaatctcag caatatgaac tagcaaagtt 301 tagcacagat caggttgttt tcatattgga gaaggtacat attttgtggg agccacttat 361 gccagcttct atttataaaa gaactatgtg cacactccta gattatgttt tttctgaaat 421 cacaaaagat atgctcctcc tagatgatat ggcagctgaa gagacattgc agcttcaaag 481 acttatccag attatgctgg agaatctatc ctcattgcta gagtcattaa ttgcagatgt 541 ggatgaaaag gaaagatatt taaagaacaa tgattgggct cggttagatg aaatggtacc 601 atcttatgca aattccgcaa acttgcagac ttatttgata tgcctctaaa atcc //