LOCUS       FX159512                 690 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Schima mertensiana mRNA, contig: Sm_rep_c13935.
VERSION     FX159512.1
DBLINK      BioProject:PRJDA67331
            Sequence Read Archive:DRR001101
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Schima mertensiana
  ORGANISM  Schima mertensiana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
            Pentapetalae; asterids; Ericales; Theaceae; Schima.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K. and Yoshimaru,H.
  TITLE     Isolation and characterization of EST-SSR markers in Schima
            mertensiana using next-generation pyrosequencing
  JOURNAL   Unpublished (2011)
COMMENT     Several-year-old seedlings of Schima mertensiana naturally grown
            in Bonin Island were used for RNA extraction. About 3 g of inner
            bark and current year twigs were cut and frozen in liquid
            nitrogen, ground using mortar and pestle. Total RNA was extracted
            by the CTAB method (Le Provost et al., 2007), and contaminated DNA
            was removed by SV total RNA isolation system (Promega, Madison,
            WI). The cDNA library construction and next generation sequencing
            by Roche 454 GS FLX sequencer with titanium chemistry were carried
            out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To
            remove polyA and adapter sequences from the cDNA sequences, the
            Program cross_match (
            phrap.html) and TIGR SeqClean sequence trimming pipeline
            ( was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..690
                     /dev_stage="several year old seedlings"
                     /note="contig: Sm_rep_c13935"
                     /organism="Schima mertensiana"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          205 a          152 c          165 g          168 t
        1 ctcaaatgca attcatctga gggttagggt ttcgtaagga actacgaaga tgtcttcgtc
       61 gaagaagatc acgctgaaga gctccgacgg agaggcgttc gaggtggagg aggtggtggc
      121 gctggaatct cagacgatca agcacatgat cgaagacgat tgcgccgaca gcgtcatacc
      181 tctccccaac gtcaccagca agatcctcgc caaggtcatc gagtattgca agaagcacgt
      241 cgagaccacc aagaacgaag atcgatctgc tgaggacgaa ctcaagactt gggacgccga
      301 gttcgtcaaa gtcgaccagc caactctatt tgatctcatt ctcgctgcta actatctgaa
      361 catcaagaac ctgttggacc ttacgtgcca ggcagttgca gacacgataa agggaaagac
      421 tccagaggaa atccgaaaga ccttcaacat caagaatgac ttcacccctg aagaagagga
      481 ggaggtccgt agggagaacc aatgggcatt tgagtaagaa attgtatggt aaaaaagcaa
      541 actctgcaaa caaacatgaa tctttaaata ttgcagctgg tgtaggtact attagggtta
      601 tgtattccgt acttttatct tcatgacttt atggttctgt ctatttaact actctcttca
      661 gtcttaatga tagcctttct tttgaattgt