LOCUS       FX158123                 673 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Schima mertensiana mRNA, contig: Sm_c12546.
ACCESSION   FX158123
VERSION     FX158123.1
DBLINK      BioProject:PRJDA67331
            Sequence Read Archive:DRR001101
            BioSample:SAMD00003860
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Schima mertensiana
  ORGANISM  Schima mertensiana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
            Pentapetalae; asterids; Ericales; Theaceae; Schima.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K. and Yoshimaru,H.
  TITLE     Isolation and characterization of EST-SSR markers in Schima
            mertensiana using next-generation pyrosequencing
  JOURNAL   Unpublished (2011)
COMMENT     Several-year-old seedlings of Schima mertensiana naturally grown
            in Bonin Island were used for RNA extraction. About 3 g of inner
            bark and current year twigs were cut and frozen in liquid
            nitrogen, ground using mortar and pestle. Total RNA was extracted
            by the CTAB method (Le Provost et al., 2007), and contaminated DNA
            was removed by SV total RNA isolation system (Promega, Madison,
            WI). The cDNA library construction and next generation sequencing
            by Roche 454 GS FLX sequencer with titanium chemistry were carried
            out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To
            remove polyA and adapter sequences from the cDNA sequences, the
            Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/
            phrap.html) and TIGR SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..673
                     /db_xref="taxon:678541"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Sm_c12546"
                     /organism="Schima mertensiana"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          154 a          120 c          170 g          229 t
ORIGIN      
        1 gttgaaggaa gcgggtattt attgcataca gggaaatggg agttgttgaa gaaggatttc
       61 tttatgcagg ctccggcaaa gttgacagct tgcgattatc atcgggggct tgatcttgtg
      121 gttgttgggt tctcaaatgg cttgtttggg ctgtatcaga tgcccgattt tatttgcatt
      181 cagttgctgt caatatcaag agagaagatt acaacggttg tttttaacga tcttgggaat
      241 tggttgacgc tcggatgtgc aaaacttggg cagttgcttg tttgggaatg gaaatcggaa
      301 agttacatct tgaaacagca gggtcattat ttcgatgtga attgtcttgc atattctcca
      361 gattcacagt tcttggctac aggagcggat gacaacaaaa tcaaggtgtg gactgtttcc
      421 tcaggcttct gttttgtgac attttcggag cacaccagtg cagtcactgc acttcatttt
      481 atggccagca accatgttct cttaagtgca tctcttgatg gaactgttcg cgcatgggat
      541 cttttccgct atcggaattt caggactttc actacccctt cttcttctaa gcaatttgtt
      601 tctttagcat cagatccgag tggtgaagtg atttgtgctg gaacttttga ttcattcgag
      661 atttatgtct ggt
//