LOCUS FX157600 732 bp mRNA linear TSA 23-MAY-2012 DEFINITION TSA: Schima mertensiana mRNA, contig: Sm_c12023. ACCESSION FX157600 VERSION FX157600.1 DBLINK BioProject:PRJDA67331 Sequence Read Archive:DRR001101 BioSample:SAMD00003860 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Schima mertensiana ORGANISM Schima mertensiana Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; asterids; Ericales; Theaceae; Schima. REFERENCE 1 (bases 1 to 732) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Setsuko,S., Uchiyama,K., Sugai,K. and Yoshimaru,H. TITLE Isolation and characterization of EST-SSR markers in Schima mertensiana using next-generation pyrosequencing JOURNAL Unpublished (2011) COMMENT Several-year-old seedlings of Schima mertensiana naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark and current year twigs were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/ phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..732 /db_xref="taxon:678541" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Sm_c12023" /organism="Schima mertensiana" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 183 a 154 c 194 g 198 t ORIGIN 1 cgcgcgcgcg cgcgagagag cgatcgtcgt cttcgggttt tcgtccgcag agtgtcttct 61 ctctcagttg tttcgagatt tgcttcccaa aatcacactc ccatctcttc tcaaaaagca 121 actgagagac agttttggag cttcctttgg tactcagtaa tggcggacga agccctgcct 181 tctcatagaa gggacccaat caagttttca gttggaaatg ttgctgcaaa tcgaagacga 241 caaaatgcag tcacagtggg aaaagaaaga agggaagcac tggtgcgrac gaagcgcttg 301 tgtagagtgg gagttagtga tgattctgtc gtcccccttg acagtgatat gatgattgat 361 gaagagcaat cagttttgga tgatcagacg ttttcagctg tggaagagct aaagcttgcc 421 attgcatatc aggggaaagg agcaatggcg aaaaagagtg gctgcccttc gtgaattaag 481 gcgtttgttg tcaagatctg aattccctcc tgttgaagct gccttaaaag ctggagctgt 541 acctctcctt gtgcagggcc tttcatttgg ttcacyagat gaacagttgc ttgaagcagc 601 ttggtgtctt acaaacatag cagcaggaaa gccagaagaa actaaagtct ttgttgactg 661 tctttaccat tgctctattg ctcatcttgg agaaagngct ccttgcctgt tgctgagcag 721 tgtgcagtgg gc //