LOCUS       FX157600                 732 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Schima mertensiana mRNA, contig: Sm_c12023.
ACCESSION   FX157600
VERSION     FX157600.1
DBLINK      BioProject:PRJDA67331
            Sequence Read Archive:DRR001101
            BioSample:SAMD00003860
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Schima mertensiana
  ORGANISM  Schima mertensiana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; asterids; Ericales; Theaceae; Schima.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K. and Yoshimaru,H.
  TITLE     Isolation and characterization of EST-SSR markers in Schima
            mertensiana using next-generation pyrosequencing
  JOURNAL   Unpublished (2011)
COMMENT     Several-year-old seedlings of Schima mertensiana naturally grown
            in Bonin Island were used for RNA extraction. About 3 g of inner
            bark and current year twigs were cut and frozen in liquid
            nitrogen, ground using mortar and pestle. Total RNA was extracted
            by the CTAB method (Le Provost et al., 2007), and contaminated DNA
            was removed by SV total RNA isolation system (Promega, Madison,
            WI). The cDNA library construction and next generation sequencing
            by Roche 454 GS FLX sequencer with titanium chemistry were carried
            out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To
            remove polyA and adapter sequences from the cDNA sequences, the
            Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/
            phrap.html) and TIGR SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..732
                     /db_xref="taxon:678541"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Sm_c12023"
                     /organism="Schima mertensiana"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          183 a          154 c          194 g          198 t
ORIGIN      
        1 cgcgcgcgcg cgcgagagag cgatcgtcgt cttcgggttt tcgtccgcag agtgtcttct
       61 ctctcagttg tttcgagatt tgcttcccaa aatcacactc ccatctcttc tcaaaaagca
      121 actgagagac agttttggag cttcctttgg tactcagtaa tggcggacga agccctgcct
      181 tctcatagaa gggacccaat caagttttca gttggaaatg ttgctgcaaa tcgaagacga
      241 caaaatgcag tcacagtggg aaaagaaaga agggaagcac tggtgcgrac gaagcgcttg
      301 tgtagagtgg gagttagtga tgattctgtc gtcccccttg acagtgatat gatgattgat
      361 gaagagcaat cagttttgga tgatcagacg ttttcagctg tggaagagct aaagcttgcc
      421 attgcatatc aggggaaagg agcaatggcg aaaaagagtg gctgcccttc gtgaattaag
      481 gcgtttgttg tcaagatctg aattccctcc tgttgaagct gccttaaaag ctggagctgt
      541 acctctcctt gtgcagggcc tttcatttgg ttcacyagat gaacagttgc ttgaagcagc
      601 ttggtgtctt acaaacatag cagcaggaaa gccagaagaa actaaagtct ttgttgactg
      661 tctttaccat tgctctattg ctcatcttgg agaaagngct ccttgcctgt tgctgagcag
      721 tgtgcagtgg gc
//