LOCUS       FX157132                1102 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Schima mertensiana mRNA, contig: Sm_rep_c11555.
ACCESSION   FX157132
VERSION     FX157132.1
DBLINK      BioProject:PRJDA67331
            Sequence Read Archive:DRR001101
            BioSample:SAMD00003860
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Schima mertensiana
  ORGANISM  Schima mertensiana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
            Pentapetalae; asterids; Ericales; Theaceae; Schima.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K. and Yoshimaru,H.
  TITLE     Isolation and characterization of EST-SSR markers in Schima
            mertensiana using next-generation pyrosequencing
  JOURNAL   Unpublished (2011)
COMMENT     Several-year-old seedlings of Schima mertensiana naturally grown
            in Bonin Island were used for RNA extraction. About 3 g of inner
            bark and current year twigs were cut and frozen in liquid
            nitrogen, ground using mortar and pestle. Total RNA was extracted
            by the CTAB method (Le Provost et al., 2007), and contaminated DNA
            was removed by SV total RNA isolation system (Promega, Madison,
            WI). The cDNA library construction and next generation sequencing
            by Roche 454 GS FLX sequencer with titanium chemistry were carried
            out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To
            remove polyA and adapter sequences from the cDNA sequences, the
            Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/
            phrap.html) and TIGR SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..1102
                     /db_xref="taxon:678541"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Sm_rep_c11555"
                     /organism="Schima mertensiana"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          318 a          233 c          232 g          314 t
ORIGIN      
        1 gcaattgctg aacacaatgt caccgtcgtt ggaatggatg ggagctacct caagcccata
       61 tccacaccaa atgtggcact atccccaggg caaacaatta acgtcctatt cacggcaaat
      121 caacctcctg atcactacta catggctgct agacagtttg ttaccgtggg ttcggctgat
      181 gttgataatg ctaccacagc agttatagaa tacagtggca actatactcc ttcgtcttcc
      241 cctgtcttcc catccaacct tcctttccac acaaacattg aggctgcaaa atgaattttt
      301 gtcccgctta aggagtttag ccagtgaaga acaccctgta tttgtgccac aaaaaaatta
      361 gtcacccaat gtttatagtt gtttctgaga aagagattgt ttgtccaaat agtacttgcg
      421 agacggcgga tggaaatagg attaagttca gccatgaaca acattacttt tctgatgcct
      481 acaacagata tattgcttgc ctactacagc aatatcagtg gggtttatga taccgatttt
      541 ccggattttc caaactggtt ttataacttc acgagtgatg atttgccaaa taatactgtg
      601 attccaacca ttggaaccaa ggtgagggtg tttgattaca atgaaacagt ggagatagtg
      661 tttcagggaa ccgatgttct ggctggagcr cagaatcacc ccatgcattt gcatgggcat
      721 agcttttatg tggttggwtc aggagttggg aatttcraca atgagacaga cccgaaatca
      781 tataatttgg ttgatccacc tgaattgaat acaatwcgag ttcctaagaa tggatgggty
      841 actgtcagat tcacgacaaa taatccaggt gtatggctgt ggcactgtca tttgctccga
      901 catctcacct ggggtatgaa aactgtcttt atagtgaaga atggtgggac tccagagacg
      961 agcctccttg atccccctcc ttccatgcct tcttgcaaag ataaatttgt caattatatc
     1021 gaggagttca aagattcaga agtcgtgcaa ccaattcgga taagtggaaa tataaacaaa
     1081 ttaagtgtaa tttgatttag cc
//