LOCUS FX145560 743 bp mRNA linear TSA 23-MAY-2012 DEFINITION TSA: Elaeocarpus photiniifolius mRNA, contig: Ep_rep_c11665. ACCESSION FX145560 VERSION FX145560.1 DBLINK BioProject:PRJDA67329 Sequence Read Archive:DRR001057 BioSample:SAMD00013926 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Elaeocarpus photiniifolius ORGANISM Elaeocarpus photiniifolius Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Oxalidales; Elaeocarpaceae; Elaeocarpus. REFERENCE 1 (bases 1 to 743) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Sugai,K., Setsuko,S., Uchiyama,K., Kato,H., Murakami,N. and Yoshimaru,H. TITLE Development of EST-SSR for Elaeocarpus photiniifolius (Elaeocarpaceae), an endemic taxon of the Bonin (Ogasawara) Islands JOURNAL Unpublished (2011) COMMENT Several-year-old seedlings of Elaeocarpus photiniifolius naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..743 /db_xref="taxon:232873" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ep_rep_c11665" /note="synonym: Elaeocarpus photiniaefolius" /organism="Elaeocarpus photiniifolius" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 215 a 159 c 182 g 187 t ORIGIN 1 gcaacataca tagcaggcac attgaaggtc tcaaacataa tttgagtcat cttttctctg 61 ttagccttag ggttaagagg tgcctctgtg agaagtacag gatgctcttc tggagcaaca 121 cgaagttcat tgtagaaggt gtgatgccaa attttctcca tgtcatccca gtctaaaaga 181 ggtatcctca ccctgaagta ccccattgag catggtatag tcagcaactg ggatgacatg 241 gtgaaaaggt tgttcaggag aagaaggtgc ttgaagttat cgtggagaag ggcatgcaaa 301 atggacagag gattacattc cctggagaag cagatgaagc gcctgacaca gttactggag 361 atattgtgtt tgttctgcaa cagaaggatc accccaagtt taagcgaaag actgatgact 421 tatttgttga acacaccttg tctctcacag aagcactctg tggcttccaa tttatattga 481 cccatctgga tggcaggcag ctcctcatta aatcacaacc tggggaagtt atcaagcctg 541 accaattcaa ggctgtaaat gatgaaggaa tgcccatata tcagaggcca ttcatgaggg 601 gaaaattgta cattcacttc acagttgatt tcccagactc cctgagccca gagcagtgca 661 aggcactgga gtctgtcctg cctccaagga cgtcggtcca attaaccgat atggagctgg 721 atgaatgcga ggagactacc ctg //