LOCUS       FX145450                 771 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Elaeocarpus photiniifolius mRNA, contig: Ep_rep_c11555.
ACCESSION   FX145450
VERSION     FX145450.1
DBLINK      BioProject:PRJDA67329
            Sequence Read Archive:DRR001057
            BioSample:SAMD00013926
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Elaeocarpus photiniifolius
  ORGANISM  Elaeocarpus photiniifolius
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Oxalidales; Elaeocarpaceae;
            Elaeocarpus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Sugai,K., Setsuko,S., Uchiyama,K., Kato,H., Murakami,N. and
            Yoshimaru,H.
  TITLE     Development of EST-SSR for Elaeocarpus photiniifolius
            (Elaeocarpaceae), an endemic taxon of the Bonin (Ogasawara) Islands
  JOURNAL   Unpublished (2011)
COMMENT     Several-year-old seedlings of Elaeocarpus photiniifolius naturally
            grown in Bonin Island were used for RNA extraction. About 3 g of
            inner bark, current year twigs and terminal buds were cut and
            frozen in liquid nitrogen, ground using mortar and pestle. Total
            RNA was extracted by the CTAB method (Le Provost et al., 2007),
            and contaminated DNA was removed by SV total RNA isolation system
            (Promega, Madison, WI). The cDNA library construction and next
            generation sequencing by Roche 454 GS FLX sequencer with titanium
            chemistry were carried out by the Hokkaido System Science Co.,
            Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from
            the cDNA sequences, the Program cross_match
            (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR
            SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..771
                     /db_xref="taxon:232873"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Ep_rep_c11555"
                     /note="synonym: Elaeocarpus photiniaefolius"
                     /organism="Elaeocarpus photiniifolius"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          228 a          145 c          215 g          183 t
ORIGIN      
        1 gtgctctcgt gtttctctgt accccatctt tacgggatct agataaataa caaaagaaaa
       61 gaaaaaatgt ttggtagagc accaaagaag agtgataact ccaagtacta tgagattctt
      121 ggagtctcaa agagcgcttc acaagatgat ctaaagaagg cttatcgtaa agctgccatc
      181 aagaaccatc ctgataaggg tggagatcct gagaagttta aagagttggc gcaagcttat
      241 gaggttctga gtgaccctga gaaacgtgag atttatgatc agtatggcga ggacgccctt
      301 aaggaaggaa tgggtggagg tggtggtgga cacgatccat tcgacatttt ccagtccttc
      361 tttggcggca acccatttgg tggtggtgga agcagcagag gacgaaggca gagaagggga
      421 gaggatgtag tccatcccct taaggtttct ttggaagatc tctacaatgg gacatccaag
      481 aagctgtctc tctctcgtaa cgtaatctgc tccaagtgca agggtaaagg atcaaagtca
      541 ggtgcatcca tgaaatgttc tggctgccaa ggttctggaa tgaaagtctc cattagacac
      601 cttggccctt cgatgatcca gcagatgcag catccttgca atgagtgtaa gggtactggt
      661 gagaccatca atgacaagga tcgctgccca cagtgcaagg gtgaaaaggt tgttcaggag
      721 aagaaggtgc ttgaagttat cgtggagaag ggcatgcaaa atggacagag t
//