LOCUS FX145450 771 bp mRNA linear TSA 23-MAY-2012 DEFINITION TSA: Elaeocarpus photiniifolius mRNA, contig: Ep_rep_c11555. ACCESSION FX145450 VERSION FX145450.1 DBLINK BioProject:PRJDA67329 Sequence Read Archive:DRR001057 BioSample:SAMD00013926 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Elaeocarpus photiniifolius ORGANISM Elaeocarpus photiniifolius Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Oxalidales; Elaeocarpaceae; Elaeocarpus. REFERENCE 1 (bases 1 to 771) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Sugai,K., Setsuko,S., Uchiyama,K., Kato,H., Murakami,N. and Yoshimaru,H. TITLE Development of EST-SSR for Elaeocarpus photiniifolius (Elaeocarpaceae), an endemic taxon of the Bonin (Ogasawara) Islands JOURNAL Unpublished (2011) COMMENT Several-year-old seedlings of Elaeocarpus photiniifolius naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..771 /db_xref="taxon:232873" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ep_rep_c11555" /note="synonym: Elaeocarpus photiniaefolius" /organism="Elaeocarpus photiniifolius" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 228 a 145 c 215 g 183 t ORIGIN 1 gtgctctcgt gtttctctgt accccatctt tacgggatct agataaataa caaaagaaaa 61 gaaaaaatgt ttggtagagc accaaagaag agtgataact ccaagtacta tgagattctt 121 ggagtctcaa agagcgcttc acaagatgat ctaaagaagg cttatcgtaa agctgccatc 181 aagaaccatc ctgataaggg tggagatcct gagaagttta aagagttggc gcaagcttat 241 gaggttctga gtgaccctga gaaacgtgag atttatgatc agtatggcga ggacgccctt 301 aaggaaggaa tgggtggagg tggtggtgga cacgatccat tcgacatttt ccagtccttc 361 tttggcggca acccatttgg tggtggtgga agcagcagag gacgaaggca gagaagggga 421 gaggatgtag tccatcccct taaggtttct ttggaagatc tctacaatgg gacatccaag 481 aagctgtctc tctctcgtaa cgtaatctgc tccaagtgca agggtaaagg atcaaagtca 541 ggtgcatcca tgaaatgttc tggctgccaa ggttctggaa tgaaagtctc cattagacac 601 cttggccctt cgatgatcca gcagatgcag catccttgca atgagtgtaa gggtactggt 661 gagaccatca atgacaagga tcgctgccca cagtgcaagg gtgaaaaggt tgttcaggag 721 aagaaggtgc ttgaagttat cgtggagaag ggcatgcaaa atggacagag t //