LOCUS FX145303 577 bp mRNA linear TSA 23-MAY-2012 DEFINITION TSA: Elaeocarpus photiniifolius mRNA, contig: Ep_c11408. ACCESSION FX145303 VERSION FX145303.1 DBLINK BioProject:PRJDA67329 Sequence Read Archive:DRR001057 BioSample:SAMD00013926 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Elaeocarpus photiniifolius ORGANISM Elaeocarpus photiniifolius Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Oxalidales; Elaeocarpaceae; Elaeocarpus. REFERENCE 1 (bases 1 to 577) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Sugai,K., Setsuko,S., Uchiyama,K., Kato,H., Murakami,N. and Yoshimaru,H. TITLE Development of EST-SSR for Elaeocarpus photiniifolius (Elaeocarpaceae), an endemic taxon of the Bonin (Ogasawara) Islands JOURNAL Unpublished (2011) COMMENT Several-year-old seedlings of Elaeocarpus photiniifolius naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..577 /db_xref="taxon:232873" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ep_c11408" /note="synonym: Elaeocarpus photiniaefolius" /organism="Elaeocarpus photiniifolius" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 181 a 109 c 110 g 177 t ORIGIN 1 gttcatacta gcaaagacat atatcctaca atcttagagg aaattgctgt tgataaatgc 61 aacgatagct gttcaaatat gtgctatatt cattacccgg caacaaaatg ttgaaaggat 121 tccagagatg gaatggcaat gttttgcagt agctccacac aaaagcaact caagtgtcaa 181 atctctgttc accatctgct gttgcctgga cttgagctgc gtactgtaag ttccaaagta 241 tgtcctcaaa agatggacgg ctccacgatt cagaagaaat gcatttgttt gttatggaga 301 tcacaattga taaagattct tgggagcaag tggccagcac aattgggttt actatccttt 361 cacgttcatc ttgactgtta aaaattgcct gtaaaatgca gcctcgagtt tcagaaattc 421 tactagtatt cacttatgat tctagggaaa ctgtcataca cattgtaact atatatggat 481 tgtttttgag tcaagagtat aagcacatac cagttcatct cgtataaatg cctctcttct 541 agtagatatt gaatgtccaa caagtgactc aagtaat //