LOCUS FX145146 915 bp mRNA linear TSA 23-MAY-2012 DEFINITION TSA: Elaeocarpus photiniifolius mRNA, contig: Ep_c11251. ACCESSION FX145146 VERSION FX145146.1 DBLINK BioProject:PRJDA67329 Sequence Read Archive:DRR001057 BioSample:SAMD00013926 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Elaeocarpus photiniifolius ORGANISM Elaeocarpus photiniifolius Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Oxalidales; Elaeocarpaceae; Elaeocarpus. REFERENCE 1 (bases 1 to 915) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Sugai,K., Setsuko,S., Uchiyama,K., Kato,H., Murakami,N. and Yoshimaru,H. TITLE Development of EST-SSR for Elaeocarpus photiniifolius (Elaeocarpaceae), an endemic taxon of the Bonin (Ogasawara) Islands JOURNAL Unpublished (2011) COMMENT Several-year-old seedlings of Elaeocarpus photiniifolius naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..915 /db_xref="taxon:232873" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ep_c11251" /note="synonym: Elaeocarpus photiniaefolius" /organism="Elaeocarpus photiniifolius" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 256 a 160 c 222 g 277 t ORIGIN 1 cagagaatgg aagcgtggcg tgcaagtgcg cgagtaaacg aagctttaac ggtttcagag 61 aagtacgttt cgaggagaag agaaaaactc gaaggaatct tcgaaacgtt tttatgattc 121 ctctctccaa aaactagttt ctttaacact aaatggcgat ccgttgcttc tggtactgca 181 accacccgca ttttaagaat ggatgcccga gagaaatcaa gatcgacgat attggaatca 241 tcgaaacata agaaggtccc catatatgtg atgatgcctg ttgatgcatt ttgcattgat 301 gcttctggaa gtcctaggat tagaaaaatc aaggccttaa ctgtatctct aaaagcactc 361 aagttggcag gtgttcatgg aattgcagtt gaggtttggt ggggaatcgt ggagcgtttt 421 tctccttttg tagtataatt ggtctctgta tgaagaactt ttcaaactga tatctgaaat 481 ggggctcaag ctgcatgttg ccttgtcttt tcactcaaac aaacactcat ttaatggaaa 541 tgggggtgtg agtttgccac tatggattac agagattggt gatagcaata aggatatata 601 ctatcgagac cgtaatgggt tctccaatga tgattatctt acactagggg tggatcaact 661 ccctctgttt tacggccgga ctgccctcca atgttatgag gacttcatgc tcagttttgt 721 aataaatttg aatcctttat tggaagtgtt attgaagaaa taagtgttgg tcttggtcct 781 tctggagaac ttaggtatcc tgcgcatcct tctggtgatg gtagatggaa tttccctgga 841 attggtgaat ttcagtgtta cgacaagtat atgatggagg acttgaagat ggctgcatgc 901 caagaaggaa accct //