LOCUS FX144462 592 bp mRNA linear TSA 23-MAY-2012 DEFINITION TSA: Elaeocarpus photiniifolius mRNA, contig: Ep_c10567. ACCESSION FX144462 VERSION FX144462.1 DBLINK BioProject:PRJDA67329 Sequence Read Archive:DRR001057 BioSample:SAMD00013926 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Elaeocarpus photiniifolius ORGANISM Elaeocarpus photiniifolius Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Oxalidales; Elaeocarpaceae; Elaeocarpus. REFERENCE 1 (bases 1 to 592) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Sugai,K., Setsuko,S., Uchiyama,K., Kato,H., Murakami,N. and Yoshimaru,H. TITLE Development of EST-SSR for Elaeocarpus photiniifolius (Elaeocarpaceae), an endemic taxon of the Bonin (Ogasawara) Islands JOURNAL Unpublished (2011) COMMENT Several-year-old seedlings of Elaeocarpus photiniifolius naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..592 /db_xref="taxon:232873" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ep_c10567" /note="synonym: Elaeocarpus photiniaefolius" /organism="Elaeocarpus photiniifolius" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 212 a 127 c 120 g 131 t ORIGIN 1 gtatttcatc tgttcatgac agacattaaa gaagctaaaa gagcactaac aaaagtataa 61 tatggacaat tcctcaatta caaaacagta aacacaaaag gcactaacag atttacaatc 121 acacaagatt gacaacccta cacatctgtt agcggttgga gccctgatag aagatataat 181 ttattgccaa agaagagcaa agagaacaaa gaaaccaata aaaaggagga gcctcttcag 241 gtaataatct tgctgtcctt gacctgtatg ctgacgatac ccactgtgca tgaggaccat 301 gaaatgaatt agaataccca taaggaaamc magcagctcc accactacat ggcagcatca 361 ggaaatccat gcacctgaaa gttaaacaac gatgggacga gaccaccaaa agcagcagac 421 aatgtgaaat tcccaaatct tgcagttgcc ataggggcaa accctcccat taatccaaac 481 ccaggttgag gaaaatgatt tggttctgga ggaggagctg tttcaggtct ctgacccgcc 541 ggacgatttg gaatattaac tccaggtatg gattttgatc tcgggtcact tg //