LOCUS FX144311 817 bp mRNA linear TSA 23-MAY-2012 DEFINITION TSA: Elaeocarpus photiniifolius mRNA, contig: Ep_c10416. ACCESSION FX144311 VERSION FX144311.1 DBLINK BioProject:PRJDA67329 Sequence Read Archive:DRR001057 BioSample:SAMD00013926 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Elaeocarpus photiniifolius ORGANISM Elaeocarpus photiniifolius Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Oxalidales; Elaeocarpaceae; Elaeocarpus. REFERENCE 1 (bases 1 to 817) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Sugai,K., Setsuko,S., Uchiyama,K., Kato,H., Murakami,N. and Yoshimaru,H. TITLE Development of EST-SSR for Elaeocarpus photiniifolius (Elaeocarpaceae), an endemic taxon of the Bonin (Ogasawara) Islands JOURNAL Unpublished (2011) COMMENT Several-year-old seedlings of Elaeocarpus photiniifolius naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..817 /db_xref="taxon:232873" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ep_c10416" /note="synonym: Elaeocarpus photiniaefolius" /organism="Elaeocarpus photiniifolius" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 218 a 216 c 140 g 243 t ORIGIN 1 caatgtcatt ggactcaccc ttggtgtcca ttaagagctt gaaagtaagg agagatggct 61 tgacactttc tttctccatc aacaataaga tgtcggctat tttctttttg ttcatcctct 121 tgtagagaat gatcatctga ttgcaagcat gaacggtgag tggtagctcc aactctgtca 181 ttttcttgag cactgcctct cctttcctca tgttgagcgc atggacacaa ctagctaaga 241 gggctcggta tactggttca cctttgcagg aaactgggat cttctcaaca tatgtctccg 301 ccatctggag gccccgtgcc ctggcaatca actcaagacg agatgcataa tcccactctg 361 taaagtcaag ttgcttactt gactccaacc actctgagaa ctgcaacgct ttcccataca 421 ctcgattgtt ccgaagatta attatggtcc tagtaatcac agttcggtcc aacttattcc 481 cggcctcaac ccacttattg agaaaattca aaatagaagg gccttgatcc ataatggcct 541 tgaacaactc ctcagaagct cttctccttt cagagtactc actactctca cccatttcag 601 tatctaataa atcattatcc aagaactctg atttagattc taactcttcc ccatcttctt 661 ccccgtcatg tatatcagct gcagcccgtg cttcgatctc tgtaaaccca tcttcaatat 721 catcattctc atcgctactt cttgtagcac ccgcctgtga acaagattac gaatcccaac 781 aaaaggtatt catgaactga tggaatatgg taaaaac //