LOCUS       FX144311                 817 bp    mRNA    linear   TSA 23-MAY-2012
DEFINITION  TSA: Elaeocarpus photiniifolius mRNA, contig: Ep_c10416.
ACCESSION   FX144311
VERSION     FX144311.1
DBLINK      BioProject:PRJDA67329
            Sequence Read Archive:DRR001057
            BioSample:SAMD00013926
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Elaeocarpus photiniifolius
  ORGANISM  Elaeocarpus photiniifolius
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Oxalidales; Elaeocarpaceae;
            Elaeocarpus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Sugai,K., Setsuko,S., Uchiyama,K., Kato,H., Murakami,N. and
            Yoshimaru,H.
  TITLE     Development of EST-SSR for Elaeocarpus photiniifolius
            (Elaeocarpaceae), an endemic taxon of the Bonin (Ogasawara) Islands
  JOURNAL   Unpublished (2011)
COMMENT     Several-year-old seedlings of Elaeocarpus photiniifolius naturally
            grown in Bonin Island were used for RNA extraction. About 3 g of
            inner bark, current year twigs and terminal buds were cut and
            frozen in liquid nitrogen, ground using mortar and pestle. Total
            RNA was extracted by the CTAB method (Le Provost et al., 2007),
            and contaminated DNA was removed by SV total RNA isolation system
            (Promega, Madison, WI). The cDNA library construction and next
            generation sequencing by Roche 454 GS FLX sequencer with titanium
            chemistry were carried out by the Hokkaido System Science Co.,
            Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from
            the cDNA sequences, the Program cross_match
            (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR
            SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..817
                     /db_xref="taxon:232873"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Ep_c10416"
                     /note="synonym: Elaeocarpus photiniaefolius"
                     /organism="Elaeocarpus photiniifolius"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          218 a          216 c          140 g          243 t
ORIGIN      
        1 caatgtcatt ggactcaccc ttggtgtcca ttaagagctt gaaagtaagg agagatggct
       61 tgacactttc tttctccatc aacaataaga tgtcggctat tttctttttg ttcatcctct
      121 tgtagagaat gatcatctga ttgcaagcat gaacggtgag tggtagctcc aactctgtca
      181 ttttcttgag cactgcctct cctttcctca tgttgagcgc atggacacaa ctagctaaga
      241 gggctcggta tactggttca cctttgcagg aaactgggat cttctcaaca tatgtctccg
      301 ccatctggag gccccgtgcc ctggcaatca actcaagacg agatgcataa tcccactctg
      361 taaagtcaag ttgcttactt gactccaacc actctgagaa ctgcaacgct ttcccataca
      421 ctcgattgtt ccgaagatta attatggtcc tagtaatcac agttcggtcc aacttattcc
      481 cggcctcaac ccacttattg agaaaattca aaatagaagg gccttgatcc ataatggcct
      541 tgaacaactc ctcagaagct cttctccttt cagagtactc actactctca cccatttcag
      601 tatctaataa atcattatcc aagaactctg atttagattc taactcttcc ccatcttctt
      661 ccccgtcatg tatatcagct gcagcccgtg cttcgatctc tgtaaaccca tcttcaatat
      721 catcattctc atcgctactt cttgtagcac ccgcctgtga acaagattac gaatcccaac
      781 aaaaggtatt catgaactga tggaatatgg taaaaac
//