LOCUS FX144123 562 bp mRNA linear TSA 23-MAY-2012 DEFINITION TSA: Elaeocarpus photiniifolius mRNA, contig: Ep_rep_c10228. ACCESSION FX144123 VERSION FX144123.1 DBLINK BioProject:PRJDA67329 Sequence Read Archive:DRR001057 BioSample:SAMD00013926 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Elaeocarpus photiniifolius ORGANISM Elaeocarpus photiniifolius Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Oxalidales; Elaeocarpaceae; Elaeocarpus. REFERENCE 1 (bases 1 to 562) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (30-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Sugai,K., Setsuko,S., Uchiyama,K., Kato,H., Murakami,N. and Yoshimaru,H. TITLE Development of EST-SSR for Elaeocarpus photiniifolius (Elaeocarpaceae), an endemic taxon of the Bonin (Ogasawara) Islands JOURNAL Unpublished (2011) COMMENT Several-year-old seedlings of Elaeocarpus photiniifolius naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..562 /db_xref="taxon:232873" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ep_rep_c10228" /note="synonym: Elaeocarpus photiniaefolius" /organism="Elaeocarpus photiniifolius" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 158 a 129 c 174 g 101 t ORIGIN 1 gagaagacag gatcgagtca gtacgcgatc accaagttca ccgaagagaa gcagaagcaa 61 ttgccttcaa attttagaaa gcttttgctt cttcatttga agaagctcgt cgcttctggt 121 aggctcgtca aggtcaaaag ctccttcaag ctccctcctc cgccaactaa gaaaccaacc 181 accaccacca agccgaaagc accggctaaa gccgtccagc cgaagcctaa aaccgccgcg 241 aaacctaagc cgaaggcacc ggctgctgcg aagccaaaac cgaaaccgaa accgaaagct 301 agtttgaagc gcaagccggc ggcgaagaag gtggctgcgc cgccgaaaac taagccgaag 361 aaggttgcgg cggcggcggc ggcggcggct aagaagccga agagtgtgaa gtcgccggcg 421 aagagaggtc gtccggcgaa gagggcgaag aagtagatag tctgttaggg gggggggggg 481 tagtgggtgt aagtttgttg gttaatgttg gtgggggtat ttaggaatag agaaatgttt 541 tagtttggat tagttgtaac cg //