LOCUS       FX130031                 325 bp    mRNA    linear   TSA 17-MAY-2014
DEFINITION  TSA: Calophyllum inophyllum mRNA, contig: Ci_c13935.
VERSION     FX130031.1
DBLINK      BioProject:PRJDA67327
            Sequence Read Archive:DRR001052
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Calophyllum inophyllum
  ORGANISM  Calophyllum inophyllum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Malpighiales; Calophyllaceae;
            Calophylleae; Calophyllum.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K., Hanaoka,S. and Yoshimaru,H.
  TITLE     Microsatellite markers derived from Calophyllum inophyllum
            (Clusiaceae) expressed sequence tags
  JOURNAL   Am. J. Bot. 99, e28-e32 (2012)
  REMARK    Publication Status: Online-Only
COMMENT     Several-year-old seedlings naturally grown in Bonin Island were
            used for RNA extraction. About 3 g of inner bark, current year
            twigs and terminal buds were cut and frozen in liquid nitrogen,
            ground using mortar and pestle. Total RNA was extracted by the
            CTAB method (Le Provost et al., 2007), and contaminated DNA was
            removed by SV total RNA isolation system (Promega, Madison, WI).
            The cDNA library construction and next generation sequencing by
            Roche 454 GS FLX sequencer with titanium chemistry were carried
            out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To
            remove polyA and adapter sequences from the cDNA sequences, the
            Program cross_match (
            phrap.html) and TIGR SeqClean sequence trimming pipeline
            ( was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..325
                     /dev_stage="several year old seedlings"
                     /note="contig: Ci_c13935"
                     /organism="Calophyllum inophyllum"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT           84 a           79 c           78 g           84 t
        1 tcacgggact agaccatctt cgctctggct ctcagcacca aaccttcatg cagtctcctc
       61 agccattcat caccaaattc cgatgatgcc ccaacaccaa cagcttatgc ttgctcagca
      121 aaacttaaca tccccatctg ctagtgatga gaatagaaga ttgagaatgc ttttgaataa
      181 ccggaatatg ggtcttggga aggatgggct tgctaactca gttggtgatg tagtcccaaa
      241 tgttggatcc cctttacagg gaggtggccc ccttttggct cgtggggagc aagatatgct
      301 gattaaggtg aaaatggctc agtgg