LOCUS FX129146 649 bp mRNA linear TSA 17-MAY-2014 DEFINITION TSA: Calophyllum inophyllum mRNA, contig: Ci_c13050. ACCESSION FX129146 VERSION FX129146.1 DBLINK BioProject:PRJDA67327 Sequence Read Archive:DRR001052 BioSample:SAMD00016598 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Calophyllum inophyllum ORGANISM Calophyllum inophyllum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Calophyllaceae; Calophylleae; Calophyllum. REFERENCE 1 (bases 1 to 649) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (26-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Setsuko,S., Uchiyama,K., Sugai,K., Hanaoka,S. and Yoshimaru,H. TITLE Microsatellite markers derived from Calophyllum inophyllum (Clusiaceae) expressed sequence tags JOURNAL Am. J. Bot. 99, e28-e32 (2012) REMARK Publication Status: Online-Only DOI:10.3732/ajb.1100299 COMMENT Several-year-old seedlings naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/ phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..649 /db_xref="taxon:158927" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ci_c13050" /organism="Calophyllum inophyllum" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 192 a 138 c 152 g 167 t ORIGIN 1 cgcatctttg tctctcctcg atttcatgct ttcggactga atcatggacg tcgatcctcg 61 taactacgaa caaatcgcta tcaaagacaa tgacatccac agcatagtac tgtcgtatct 121 ggttcataat tgttataggg aaaccgtgga atcattcgct gcttgcactg gaatgaggca 181 gcctgcagat tatctcgagg acacggagaa acggaaaagg aatcactcat tacgctttag 241 aagacaatgc actcaaggcc attgaactga ccgaagagct ggcacagggc ttactagaga 301 ataatgcaga cttgcatttt gaactcttaa gccttcattt tgtagagctt atctgcagca 361 ggaagagcac agaagcattg gagtttgctc agactaagtt gggtcctttt ggggaggtgc 421 cgaagtatgt tgaaaaactt caggacttaa tgactttgct tgcttttgag gaaccggaaa 481 agtcccccaa tgtttcatct cctgagcagg gactatcggc aagatgttgc agatagttta 541 aaccgagcta ttcttgcaca tgcaaaccgg ccaagctata ctgcaatgga gaggctgata 601 caacaaacaa ctgtggtcag gcaatgctta agtaaagact atatcaagg //