LOCUS FX128267 554 bp mRNA linear TSA 17-MAY-2014 DEFINITION TSA: Calophyllum inophyllum mRNA, contig: Ci_c12171. ACCESSION FX128267 VERSION FX128267.1 DBLINK BioProject:PRJDA67327 Sequence Read Archive:DRR001052 BioSample:SAMD00016598 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Calophyllum inophyllum ORGANISM Calophyllum inophyllum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Calophyllaceae; Calophylleae; Calophyllum. REFERENCE 1 (bases 1 to 554) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (26-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Setsuko,S., Uchiyama,K., Sugai,K., Hanaoka,S. and Yoshimaru,H. TITLE Microsatellite markers derived from Calophyllum inophyllum (Clusiaceae) expressed sequence tags JOURNAL Am. J. Bot. 99, e28-e32 (2012) REMARK Publication Status: Online-Only DOI:10.3732/ajb.1100299 COMMENT Several-year-old seedlings naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/ phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..554 /db_xref="taxon:158927" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ci_c12171" /organism="Calophyllum inophyllum" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 113 a 150 c 171 g 120 t ORIGIN 1 tacctcgccg accttctccc cgcgaccgtc caccggatca tctacttcga ctccgacctc 61 atcctcgtcg atgacgtggc caagctctgg aacatcaacc tgaactccca cgtgctgggt 121 gcacccgagt actgccacgc caatttcacc agttacttca acaatcgctt ctggtcgaac 181 ccggtgcacg cgacgtcgtt catggggaag aggccgtgct acttcaacac gggagtgatg 241 gtgatcgact tagacgcgtg gcgggcgggg aaatataccg acaggttgga gtactggatg 301 aaggtgcaga agaagtacag aatctacgag ctggggtcgc tacccccatt tctgttggtg 361 tttgcgggtg acgtggaggg ggtggagcac agatggaacc agcacgggct tggaggagat 421 aacttgcatg gactgtgtag ggatctccat cctgggcctg ttagcctgct tcattggagt 481 gggaaaggga gccctggctc aggctcgact ccaagaggcc gtgtgctttg gattcgctcg 541 tgggcgccgt acga //