LOCUS       FX128119                 837 bp    mRNA    linear   TSA 17-MAY-2014
DEFINITION  TSA: Calophyllum inophyllum mRNA, contig: Ci_c12023.
ACCESSION   FX128119
VERSION     FX128119.1
DBLINK      BioProject:PRJDA67327
            Sequence Read Archive:DRR001052
            BioSample:SAMD00016598
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Calophyllum inophyllum
  ORGANISM  Calophyllum inophyllum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Malpighiales; Calophyllaceae;
            Calophylleae; Calophyllum.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K., Hanaoka,S. and Yoshimaru,H.
  TITLE     Microsatellite markers derived from Calophyllum inophyllum
            (Clusiaceae) expressed sequence tags
  JOURNAL   Am. J. Bot. 99, e28-e32 (2012)
  REMARK    Publication Status: Online-Only
            DOI:10.3732/ajb.1100299
COMMENT     Several-year-old seedlings naturally grown in Bonin Island were
            used for RNA extraction. About 3 g of inner bark, current year
            twigs and terminal buds were cut and frozen in liquid nitrogen,
            ground using mortar and pestle. Total RNA was extracted by the
            CTAB method (Le Provost et al., 2007), and contaminated DNA was
            removed by SV total RNA isolation system (Promega, Madison, WI).
            The cDNA library construction and next generation sequencing by
            Roche 454 GS FLX sequencer with titanium chemistry were carried
            out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To
            remove polyA and adapter sequences from the cDNA sequences, the
            Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/
            phrap.html) and TIGR SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..837
                     /db_xref="taxon:158927"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Ci_c12023"
                     /organism="Calophyllum inophyllum"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          295 a          115 c          264 g          163 t
ORIGIN      
        1 gggatgggtc ccccagcaga agaaatcgtc gaggagactg aagaggaaag aagccaggca
       61 agctaaaaaa taagaggaag tgccactctt ggctcgagaa ccagagagag agaaaaatta
      121 ggcaacagga actagacaag gaaagtttag agaattcaaa gggaaatcat gagaagaagc
      181 catcaaacat acttgccaga cagaataatt catcaaaaag ccatcgaatc agaaattggg
      241 agattgggag cgtaggaaaa tgttgtatga ggctagttcg gctgagaata atgattcagg
      301 aaaaatgggt atggggaaga aaccagggat ttgtgaagga gaaaaagagt gggaagagga
      361 aaggtaagac aaaagtttga ggagtatttg ggagatggat atggcggttg ctgataggct
      421 tgcaaaggag gatttggaga tggagaggag acttgctaag agactcaagg tgaaagatgg
      481 gaaactgggg gggcttggat gatgggatca atatgttgtt ccaggggatc ccttcactgc
      541 ttggttcttc caaggatgaa gaaggaagcc ccagtgccaa agactgccct gttgaggggt
      601 ttgatgatgg aaattctcag gatgaggctg aaaattatgt tgtgggtgca ttttcagaac
      661 aggaggagat gtctggtgga gagctggggc aaggagaaga tattgctgat gctaccaaaa
      721 agaagagacc gaggaagaaa aagaagtcaa agaaaaagca tgaaggcagc ttgacaggtg
      781 ataatgctga tggcacatct ttaccagcag aaactcccga tcaagagggt acattgc
//