LOCUS FX127884 387 bp mRNA linear TSA 17-MAY-2014 DEFINITION TSA: Calophyllum inophyllum mRNA, contig: Ci_c11788. ACCESSION FX127884 VERSION FX127884.1 DBLINK BioProject:PRJDA67327 Sequence Read Archive:DRR001052 BioSample:SAMD00016598 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Calophyllum inophyllum ORGANISM Calophyllum inophyllum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Calophyllaceae; Calophylleae; Calophyllum. REFERENCE 1 (bases 1 to 387) AUTHORS Yoshimaru,H. and Suzuki,S. TITLE Direct Submission JOURNAL Submitted (26-MAY-2011) to the DDBJ/EMBL/GenBank databases. Contact:Hiroshi Yoshimaru Forestry and Forest Products Research Institute, Department of Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan REFERENCE 2 AUTHORS Setsuko,S., Uchiyama,K., Sugai,K., Hanaoka,S. and Yoshimaru,H. TITLE Microsatellite markers derived from Calophyllum inophyllum (Clusiaceae) expressed sequence tags JOURNAL Am. J. Bot. 99, e28-e32 (2012) REMARK Publication Status: Online-Only DOI:10.3732/ajb.1100299 COMMENT Several-year-old seedlings naturally grown in Bonin Island were used for RNA extraction. About 3 g of inner bark, current year twigs and terminal buds were cut and frozen in liquid nitrogen, ground using mortar and pestle. Total RNA was extracted by the CTAB method (Le Provost et al., 2007), and contaminated DNA was removed by SV total RNA isolation system (Promega, Madison, WI). The cDNA library construction and next generation sequencing by Roche 454 GS FLX sequencer with titanium chemistry were carried out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To remove polyA and adapter sequences from the cDNA sequences, the Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/ phrap.html) and TIGR SeqClean sequence trimming pipeline (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to further analyses. We used a EST sequence assembler MIRA (Chevreux et al., 2004) to run de novo assemblies. FEATURES Location/Qualifiers source 1..387 /db_xref="taxon:158927" /dev_stage="several year old seedlings" /mol_type="mRNA" /note="contig: Ci_c11788" /organism="Calophyllum inophyllum" /tissue_type="mixture of inner bark, current year twigs and terminal buds" BASE COUNT 113 a 60 c 89 g 125 t ORIGIN 1 tagttgtatg tgaaatgtcc atatttcaaa atttgctagt atgctcacta attttcaaat 61 attgctaaat tatttgcttt caggatgcaa aggaacatct tgctgcactg gctgacaagt 121 tgggttcatc attgacagat atagatttgt ctgtgaaatc cccacaaata tcagcagatg 181 ctgcagaaca agattatgat gaaaaggctg aagatgctgt tgattatgaa gacattgatg 241 agcagtatga ggggcctgag atacaagctg ctggtgagga ggactattta ttgcccaaaa 301 agatgtattt tgcttctcaa gtttctcagg cgagattgga tcctgcccct tctgtctttt 361 atgatgataa ttatgatgag ttggagg //