LOCUS       FX127651                1011 bp    mRNA    linear   TSA 17-MAY-2014
DEFINITION  TSA: Calophyllum inophyllum mRNA, contig: Ci_c11555.
ACCESSION   FX127651
VERSION     FX127651.1
DBLINK      BioProject:PRJDA67327
            Sequence Read Archive:DRR001052
            BioSample:SAMD00016598
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Calophyllum inophyllum
  ORGANISM  Calophyllum inophyllum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Malpighiales; Calophyllaceae;
            Calophylleae; Calophyllum.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Yoshimaru,H. and Suzuki,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Hiroshi Yoshimaru
            Forestry and Forest Products Research Institute, Department of
            Forest Genetics; 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan
REFERENCE   2
  AUTHORS   Setsuko,S., Uchiyama,K., Sugai,K., Hanaoka,S. and Yoshimaru,H.
  TITLE     Microsatellite markers derived from Calophyllum inophyllum
            (Clusiaceae) expressed sequence tags
  JOURNAL   Am. J. Bot. 99, e28-e32 (2012)
  REMARK    Publication Status: Online-Only
            DOI:10.3732/ajb.1100299
COMMENT     Several-year-old seedlings naturally grown in Bonin Island were
            used for RNA extraction. About 3 g of inner bark, current year
            twigs and terminal buds were cut and frozen in liquid nitrogen,
            ground using mortar and pestle. Total RNA was extracted by the
            CTAB method (Le Provost et al., 2007), and contaminated DNA was
            removed by SV total RNA isolation system (Promega, Madison, WI).
            The cDNA library construction and next generation sequencing by
            Roche 454 GS FLX sequencer with titanium chemistry were carried
            out by the Hokkaido System Science Co., Ltd. (Hokkaido, Japan). To
            remove polyA and adapter sequences from the cDNA sequences, the
            Program cross_match (http://bozeman.mbt.washington.edu/phrap.docs/
            phrap.html) and TIGR SeqClean sequence trimming pipeline
            (http://compbio.dfci.harvard.edu/tgi/software/) was used prior to
            further analyses. We used a EST sequence assembler MIRA (Chevreux
            et al., 2004) to run de novo assemblies.
FEATURES             Location/Qualifiers
     source          1..1011
                     /db_xref="taxon:158927"
                     /dev_stage="several year old seedlings"
                     /mol_type="mRNA"
                     /note="contig: Ci_c11555"
                     /organism="Calophyllum inophyllum"
                     /tissue_type="mixture of inner bark, current year twigs
                     and terminal buds"
BASE COUNT          284 a          219 c          252 g          256 t
ORIGIN      
        1 aacaacaaca tcccggtggg aacttccacc gggggggggc actgccgcca ccgccaccgc
       61 agatgatgcg gcaggagtcg gtggcttcga ctgcgttcaa cccgcaggat taccaccagc
      121 agcaacagca tcacccttcc gccccagggc gccccatcac ggtccttatg atgcatatgg
      181 tgatggtttt ggtgggagag aatgagaaag cctacccaaa ggagagcagt tgattataca
      241 agcacaattg ttcgttacat tcagaccagg atgtggcagc gagattccaa ggacaggaca
      301 gcattacagc caaccccagc tgctgctgtt gatttcttgc cacctgttgc ttattctgat
      361 aacccatcaa ccagctttgc tgcaaagttt gttcatacgt ctctaaacaa aaaccggtgt
      421 tcaattaatc aagttctgtg gactcctaat gggagacgtc taatcacagg ctctcaaagt
      481 ggggagttca ctctttggaa tggccaatca tttaattttg aaatgattct tcaggcccat
      541 gatcaagcaa tcaggtctat gatatggagc cacaatgata attggatggt gtctggtgat
      601 gatgggggtg caataaagta ttggcagagc aacatgaata atgtcaaggc aaataaatcc
      661 gcccataaag aatctgttcg tggtttgagc ttctgtagaa cagatttgaa attctgctcc
      721 tgctcggatg acactactgt taaagtttgg gactttgcta ggtgtcagga agagcgttca
      781 ttaactggcc atggttggga tgtgaaaagt gttgactggc accctacaaa atctcttcta
      841 gtttcaggtg ggaaagacaa ccttgtaaaa ctctgggatg ctaaaagtgg aagggagctg
      901 tgctcatttc atggacacaa aaatacagtg ctatgtgtca agtggaacca aaatggtaac
      961 tgggtgctaa ctgcttctaa ggatcagata atcaagctct atgatataag g
//