LOCUS FM212242 4601 bp DNA circular SYN 15-JUL-2010
DEFINITION Cloning shuttle vector pA13.
ACCESSION FM212242
VERSION FM212242.1
KEYWORDS .
SOURCE Cloning shuttle vector pA13
ORGANISM Cloning shuttle vector pA13
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4601)
AUTHORS Kojic M.
JOURNAL Submitted (24-SEP-2008) to the INSDC. Kojic M., Lmgim, Institute of
Molecular Genetics and Gene, Vojvode Stepe 444/a,11010 Belgrade,
Serbia, 11010, SERBIA AND MONTENEGRO.
REFERENCE 2
AUTHORS Kojic M., Lozo J., Jovcic B., Strahinic I., Fira D., Topisirovic L.
TITLE Construction of a new shuttle vector and its use for cloning and
expression of two plasmid-encoded bacteriocins from Lactobacillus
paracasei subsp. paracasei BGSJ2-8
JOURNAL Int. J. Food Microbiol. 140(2-3), 117-124(2010).
PUBMED 20439125
FEATURES Location/Qualifiers
source 1..4601
/organism="Cloning shuttle vector pA13"
/focus
/lab_host="E.coli, Lactococcus lactis and Lactobacillus
sp."
/mol_type="other DNA"
/note="pA13 is a derivative of the vectors M13 mp18,
pIL253 and pA1"
/db_xref="taxon:563905"
source 1..972
/organism="Escherichia coli"
/mol_type="other DNA"
/note="Cloning vector M13 mp18, position 5913-6884, INSDC
accession X02513"
/db_xref="taxon:562"
source 973..1584
/organism="Lactococcus lactis"
/mol_type="other DNA"
/note="Cloning vector pIL253, position 2821-2211, INSDC
accession AF041239"
/db_xref="taxon:1358"
source 1585..2699
/organism="Lactococcus lactis"
/mol_type="other DNA"
/note="Cloning vector pIL253, position 4327-3216, INSDC
accession AF041239"
/db_xref="taxon:1358"
source 2700..4601
/organism="Lactobacillus plantarum"
/mol_type="other DNA"
/note="natural plasmid pA1, position 1900-1, INSDC
accession Z11717.1"
/db_xref="taxon:1590"
CDS 310..810
/transl_table=11
/gene="lacZ"
/product="beta galactosidase"
/protein_id="CAR79029.1"
/translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRL
AAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVV
VPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIF
DGVPIG"
misc_difference 975..976
/replace=""
/note="insertion of TA to ablate MCS"
CDS complement(1696..2433)
/transl_table=11
/gene="ermAM"
/product="adenine methylase"
/protein_id="CAR79030.1"
/translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
KVNNLSTVTYEQVLSIFNSYLLFNGRK"
misc_difference 2689..2691
/replace=""
/note="insertion of TGT to ablate MCS"
CDS complement(2821..3411)
/transl_table=11
/gene="repB"
/product="replication protein"
/protein_id="CAR79031.1"
/translation="MTKKKYEYEKGRDWTFIVYPESAPDNWRTVLDETHLLWIESPLH
DKDMNADGEIKKSHYHILLTFDGPVTEKQVIKLIDPLNTPLLKKLGCPRWFSVVIWHI
WTTLKNINTQRDEIVGHCGADVESYFELTKTSKMSVMKTIITYIYEIRLIIMLIFNDL
YSNILMIGLTYRLTTTTLAINKMIDGMWLKKKNELR"
CDS complement(3487..3630)
/transl_table=11
/gene="repA"
/product="replication protein"
/protein_id="CAR79032.1"
/translation="MERVKVGITLTEDTLARLEEICKEMGLSKSQALSMLVNKEYLEK
ISR"
BASE COUNT 1311 a 983 c 722 g 1585 t
ORIGIN
1 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
61 ctcgctggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
121 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
181 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
241 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
301 gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc
361 aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
421 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
481 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
541 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
601 gccgatacgg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
661 accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
721 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
781 attatttttg atggcgttcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
841 cgaattttaa caaaatatta acgtttacaa tttaaatatt tgcttataca atcttcctgt
901 ttttggggct tttctgatta tcaaccgggg tacatatgat tgacatgcta gttttacgat
961 taccgttcat cgaatattct atttaatcac tttgactagc aaatactaac aacaagacac
1021 acacaccaaa aatcaaaaat tcactacttt tagttaaaaa ccacgtaacc acaagaacta
1081 atccaatcca tgtaatcggg ttcttcaaat atttctccaa gattttcctc ctctaatatg
1141 ctcaacttaa atgacctatt caataaatct attatgctgc taaatagttt ataggacaaa
1201 taagtatact ctaatgacct ataaaagata gaaaattaaa aaatcaagtg ttcgcttcgc
1261 tctcactgcc cctcgacgtt ttagtagcct ttccctcact tcgttcagtc caagccaact
1321 aaaagttttc gggctactct ctccttctcc ccctaataat taattaaaat cttactctgt
1381 atatttctgc taatcattcg ctaaacagca aagaaaaaac aaacacgtat catagatata
1441 aatgtaatgg catagtgcgg gttttatttt cagcctgtat catagctaaa caaatcgagt
1501 tgtgtgtccg ttttagggcg ttctgctagc ttgtttaaag tctcttgaat gaatgtatgc
1561 tctaagtcaa aagaatttgt cagcgctagg gacctcttta gctccttgga agctgtcagt
1621 agtataccta ataatttatc tacattccct ttagtaacgt gtaactttcc aaatttacaa
1681 aagcgactca tagaattatt tcctcccgtt aaataataga taactattaa aaatagacaa
1741 tacttgctca taagtaacgg tacttaaatt gtttactttg gcgtgtttca ttgcttgatg
1801 aaactgattt ttagtaaaca gttgacgata ttctcgattg acccattttg aaacaaagta
1861 cgtatatagc ttccaatatt tatctggaac atctgtggta tggcgggtaa gttttattaa
1921 gacactgttt acttttggtt taggatgaaa gcattccgct ggcagcttaa gcaattgctg
1981 aatcgagact tgagtgtgca agagcaaccc tagtgttcgg tgaatatcca aggtacgctt
2041 gtagaatcct tcttcaacaa tcagatagat gtcagacgca tggctttcaa aaaccacttt
2101 tttaataatt tgtgtgctta aatggtaagg aatactccca acaattttat acctctgttt
2161 gttagggaat tgaaactgta gaatatcttg gtgaattaaa gtgacacgag tattcagttt
2221 taatttttct gacgataagt tgaatagatg actgtctaat tcaatagacg ttacctgttt
2281 acttatttta gccagtttcg tcgttaaatg ccctttacct gttccaattt cgtaaacggt
2341 atcggtttct tttaaattca attgttttat tatttggttg agtacttttt cactcgttaa
2401 aaagttttga gaatatttta tatttttgtt catgtaatca ctccttctta attacaaatt
2461 tttagcatct aatttaactt caattcctat tatacaaaat tttaagatac tgcactatca
2521 acacactctt aagtttgctt ctaagtctta tttccataac ttcttttacg tttccgccat
2581 tctttgctgt ttcgattttt atgatatggt gcaagtcagc acgaacacga accgtcttat
2641 ctcccattat atcttttttt gcactgattg gtgtatcatt tcgtttttct ttttgtgcgc
2701 acccttatgg tgcaattagc ttgatacctg tttgacgatt tgctgcccta ctttttcagt
2761 aggtatgcaa acctcaaacc aaaataaaaa cccccttcga ctttcgtcag ggggctttta
2821 ttatctaagt tcatttttct tttttaacca cataccgtca atcatcttat tgattgctaa
2881 cgttgttgta gttaatcggt acgtcaaacc aatcatcaga atgtttgaat acaaatcatt
2941 aaaaatcagc ataattatca atcttatttc gtaaatataa gtgataatcg tcttcataac
3001 gctcattttg cttgttttag ttaattcaaa ataactctcc acatctgcac cacaatgccc
3061 aacaatctca tctctttgag tattgatatt tttcagggtt gtccaaatgt gccatataac
3121 gacactaaac caacgtggac aacccaactt ttttagaagt ggtgtattta atggatcaat
3181 caacttaatc acttgttttt cagtaactgg accatcaaaa gttaataaaa tatgataatg
3241 agattttttt atttcaccat ctgcattcat atctttatca tgcaaaggac tttcaatcca
3301 aagtaaatga gtttcatcca atacagttct ccaattgtct ggtgcgcttt ctggatatac
3361 aataaacgtc cagtcacgac ccttttcata ttcatatttt ttcttcgtca tgttatacta
3421 ctcctatacc gttagttatt tcgccattac cagtggcgat ttttttatgc cttaaaaacc
3481 ttattttcat cttgatattt tttctaaata ctctttatta accaacatag ataaagcttg
3541 agatttactt agccccattt ctttacaaat ttcttctagt ctagctaacg tatcttcagt
3601 caaagttata ccaaccttta ctctttccat atccatctcc ccgttctaat ttataaatat
3661 ataatactct attattctgt ataagtctag agattataca aaaaatagaa tactattaat
3721 ttttttagaa acgttattat agctataaac attgatataa caacgtttct aatttttttc
3781 acattgcaca ttgcactcct ggggggtgtc gtagtgcccc ccagaagttg gtcgattttt
3841 tcaaaatctc ccccccagtt tgcttcccaa ttcctaaaaa caataagacg gctttgccca
3901 cacccacaaa cgcaatgcgt ttgatcatgc ctctgaaaag tagctttatc acgtaattac
3961 tttttcctct cagcaaaagc taaatgccta aaataaaaag acaagggtaa accgttgctc
4021 acgttcgttc ccccttgtct ttattattta cagccatttt taacgcttct gccgacgaaa
4081 agtaataatc acgtgtaaat atactcgata tgatttaaaa cgtcttagaa cgcaaatatg
4141 agcttctaac gacttaattc atctctatct cgtgttctag tttcatttct atgaattttt
4201 tcaaaatctc caccttttac aaactcttta atttcgctca caaggttatt catgagcttt
4261 ttagcttgtc caacagttgc accaagggta ttttccataa actgattaaa ccctttgtaa
4321 atgcccttaa tttcgttttt aagcgattta ttctctttct ccaagcaatc aattttctct
4381 aaattattat taaatctatc tgccaaatca ttgtaatcac gcactacttc cttgtatttt
4441 tcttttacat agtcaatccg tttaggtaaa tcagtttctg catatttttt tagcttttca
4501 ttcgcattat atagctcttc atagctttta atcactgcat ttaatttact ttcagggata
4561 attacattgc ctgtttttcg ttcttcattt ttcatgaatt g
//