LOCUS FD483325 664 bp mRNA linear EST 30-JUN-2009 DEFINITION HA_MX2_35f02_SP6 Lobster Multiple Tissues, Normalized Homarus americanus cDNA clone HA_MX2_35f02 5' similar to ref|XP_001601602.1| PREDICTED: similar to ENSANGP00000014060 - Nasonia vitripennis. Score = 127 bits (319), Expect = 5e-28, mRNA sequence. ACCESSION FD483325 VERSION FD483325.1 DBLINK BioSample: SAMN00152457 KEYWORDS EST. SOURCE Homarus americanus (American lobster) ORGANISM Homarus americanus Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Astacidea; Nephropoidea; Nephropidae; Homarus. REFERENCE 1 (bases 1 to 664) AUTHORS Towle,D.W., Smith,C.M. and Beale,K.M. TITLE Expressed Sequence Tags in a normalized cDNA library prepared from multiple tissues of adult intermolt and postmolt American lobster, Homarus americanus JOURNAL Unpublished COMMENT Contact: Christine M. Smith Marine DNA Sequencing and Analysis Facility Mount Desert Island Biological Laboratory Old Bar Harbor Road, Salsbury Cove, ME 04672, USA Tel: 207-288-9880 x130 Fax: 207-288-2130 Email: cmsmith@mdibl.org Plate: 35 row: f column: 02 Seq primer: SP6 High quality sequence stop: 663. FEATURES Location/Qualifiers source 1..664 /organism="Homarus americanus" /mol_type="mRNA" /db_xref="taxon:6706" /clone="HA_MX2_35f02" /tissue_type="Gill, epipodite, branchiostegite, heart, ovary, testis, antennal gland, abdominal muscle, hepatopancreas, brain" /clone_lib="SAMN00152457 Lobster Multiple Tissues, Normalized" /dev_stage="Adult intermolt and postmolt" /note="Vector: pCMV Sport 6.1; Total RNA samples were prepared individually from each tissue, checked for quality, then pooled for construction and normalization of cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at Mount Desert Island Biological Laboratory. Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software)." BASE COUNT 214 a 156 c 141 g 153 t ORIGIN 1 gcagggaggc gttgtgatgg gcgcgtacta acgcaaatgc caaaatggag gatggacttc 61 taagcctcaa gtggaacaac cacaaaatta cattctttga aatcctcagg gtacttagag 121 aaaaggccaa ctttacagat gctacgattg cggtggaagg aaagttctat ccagttcata 181 aactggttat gagcacatgc agtgaatatt ttagtgaaat ttttgaaaga acaccgtgca 241 aatctccagt catagtgctg aaagatgtgc gaagtcaaga catggatgcg ttattggatt 301 acatgtactt aggtgaagtg aatgtgaacc aaaatgactt agcttcctta ttgaagacag 361 ccgagtgcct aagaattaag ggcctcgcgg tacctgatga agaccccaca aagtctagga 421 aagcgccgtc tgatgacagg agagaaagtc caccaccaaa gagaagacgg caggaagacc 481 cccctgcacc agccccacct ccaaggccag tttccccaaa catttcagca tcttcaaaaa 541 ctacaccttc aactcaaacg tctccactac aacaccaatc ttcctcttta tcagtttctc 601 atcaccaaga caatgtacaa gattcagtct tagacccccc tcctatggtt aaagttgaaa 661 tgga //