LOCUS FD483323 710 bp mRNA linear EST 30-JUN-2009 DEFINITION HA_MX2_35e12_SP6 Lobster Multiple Tissues, Normalized Homarus americanus cDNA clone HA_MX2_35e12 5', mRNA sequence. ACCESSION FD483323 VERSION FD483323.1 DBLINK BioSample: SAMN00152457 KEYWORDS EST. SOURCE Homarus americanus (American lobster) ORGANISM Homarus americanus Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Astacidea; Nephropoidea; Nephropidae; Homarus. REFERENCE 1 (bases 1 to 710) AUTHORS Towle,D.W., Smith,C.M. and Beale,K.M. TITLE Expressed Sequence Tags in a normalized cDNA library prepared from multiple tissues of adult intermolt and postmolt American lobster, Homarus americanus JOURNAL Unpublished COMMENT Contact: Christine M. Smith Marine DNA Sequencing and Analysis Facility Mount Desert Island Biological Laboratory Old Bar Harbor Road, Salsbury Cove, ME 04672, USA Tel: 207-288-9880 x130 Fax: 207-288-2130 Email: cmsmith@mdibl.org Plate: 35 row: e column: 12 Seq primer: SP6 High quality sequence stop: 709. FEATURES Location/Qualifiers source 1..710 /organism="Homarus americanus" /mol_type="mRNA" /db_xref="taxon:6706" /clone="HA_MX2_35e12" /tissue_type="Gill, epipodite, branchiostegite, heart, ovary, testis, antennal gland, abdominal muscle, hepatopancreas, brain" /clone_lib="SAMN00152457 Lobster Multiple Tissues, Normalized" /dev_stage="Adult intermolt and postmolt" /note="Vector: pCMV Sport 6.1; Total RNA samples were prepared individually from each tissue, checked for quality, then pooled for construction and normalization of cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at Mount Desert Island Biological Laboratory. Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software)." BASE COUNT 194 a 137 c 161 g 218 t ORIGIN 1 gggtggctga ggggaggaca gttggagatg taggggaggg gtgcgtggcc tggctgaatc 61 gctcacaaac tcgtctctgc acatctggat gtgggaagaa agggggttgt gcacaccctg 121 gctgagtggc tcagacatgt ctctgtcacg agaaacgtca cagaccatgt cactgcttgt 181 tccgcctact cggctgggta aactgagcaa cactgtggcc aagttagact cgtcacagca 241 accaccaggc aggctactgg gccaagctgt gaggtgttat ggctgttttt caactcatat 301 ttttgggtag ataaacgtaa ttttctttat ttatttttac tcatttacat gcacacacca 361 tcctaaatac atattgtaaa aaagtaaaag aaataattgt cctaagggca aacctgtact 421 caaatgcagt aattaatgta gttcatttga gtacaggttg tgacattgta atgattttta 481 cagaactttt atagcattac tgttgacttt taatagttgt ggaacatatc ctaattttcc 541 ccataagatt aggtattcac tatatgtgtc ccgaaccatc aaagatgcca gactattggg 601 tcctggatta acacagtcca acctgtatac caatgggtct tagcgtttgc ctactaggat 661 tatgtaattt ttttagaatt ttgatttttg ctgtacacat cgttaaaagc //