LOCUS       FD483321                 629 bp    mRNA    linear   EST 30-JUN-2009
DEFINITION  HA_MX2_35e10_SP6 Lobster Multiple Tissues, Normalized Homarus
            americanus cDNA clone HA_MX2_35e10 5' similar to
            ref|XP_001620378.1| hypothetical protein NEMVEDRAFT_v1g148340 -
            Nematostella vectensis. Score = 147 bits (372), Expect = 3e-34,
            mRNA sequence.
ACCESSION   FD483321
VERSION     FD483321.1
DBLINK      BioSample: SAMN00152457
KEYWORDS    EST.
SOURCE      Homarus americanus (American lobster)
  ORGANISM  Homarus americanus
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea;
            Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda;
            Pleocyemata; Astacidea; Nephropoidea; Nephropidae; Homarus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Towle,D.W., Smith,C.M. and Beale,K.M.
  TITLE     Expressed Sequence Tags in a normalized cDNA library prepared from
            multiple tissues of adult intermolt and postmolt American lobster,
            Homarus americanus
  JOURNAL   Unpublished
COMMENT     Contact: Christine M. Smith
            Marine DNA Sequencing and Analysis Facility
            Mount Desert Island Biological Laboratory
            Old Bar Harbor Road, Salsbury Cove, ME 04672, USA
            Tel: 207-288-9880 x130
            Fax: 207-288-2130
            Email: cmsmith@mdibl.org
            Plate: 35  row: e  column: 10
            Seq primer: SP6.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Homarus americanus"
                     /mol_type="mRNA"
                     /db_xref="taxon:6706"
                     /clone="HA_MX2_35e10"
                     /tissue_type="Gill, epipodite, branchiostegite, heart,
                     ovary, testis, antennal gland, abdominal muscle,
                     hepatopancreas, brain"
                     /clone_lib="SAMN00152457 Lobster Multiple Tissues,
                     Normalized"
                     /dev_stage="Adult intermolt and postmolt"
                     /note="Vector: pCMV Sport 6.1; Total RNA samples were
                     prepared individually from each tissue, checked for
                     quality, then pooled for construction and normalization of
                     cDNA library by Invitrogen. Plasmids were isolated and
                     inserts end-sequenced by the Marine DNA Sequencing and
                     Analysis Facility at Mount Desert Island Biological
                     Laboratory. Traces were processed for submission to dbEST
                     by trace2dbest software (Parkinson, Anthony and Blaxter,
                     unpublished software)."
BASE COUNT          109 a          198 c          153 g          169 t
ORIGIN      
        1 atcatgtgtg agatatgagg aactctctct ctctctatct tattgtggcc actacacctg
       61 ctgtggccac tacacctgct gtggccgata cacctgctgt ggccgataca cctgctgtgg
      121 ccgatacacc tgctgtggcc gatacacctg ctgtggccac tacacctgct gtggccacta
      181 cacctgctgt ggccactaca cctgctgtag ccactacagc tcttgtggcc gatacacctg
      241 ctgtggccac taaacctgct gtggccatta cacctgttgt ggccacatct tttgtggcca
      301 ctacacctgc tgtggacact acacctattg tgcccactac ttctggtgtg cccactacag
      361 ctcttgtggc cgttacagct cttgtggccg ttacagctct tgtgggcact acagctcttg
      421 tgggcactac agctcttgtg ggcactacag ctcttgtggg cactacagct cttgtgggca
      481 ctacagctct tgtgggcact acagctcttg tgggcactac agctcttgtg ggcactacag
      541 ctctgcggcc actacagctc ttgcggccgc tctagagtat ccctcgaggg gcccaagctt
      601 acgcgtaccc agctttcttg tacaagtgg
//