LOCUS FD483321 629 bp mRNA linear EST 30-JUN-2009 DEFINITION HA_MX2_35e10_SP6 Lobster Multiple Tissues, Normalized Homarus americanus cDNA clone HA_MX2_35e10 5' similar to ref|XP_001620378.1| hypothetical protein NEMVEDRAFT_v1g148340 - Nematostella vectensis. Score = 147 bits (372), Expect = 3e-34, mRNA sequence. ACCESSION FD483321 VERSION FD483321.1 DBLINK BioSample: SAMN00152457 KEYWORDS EST. SOURCE Homarus americanus (American lobster) ORGANISM Homarus americanus Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Astacidea; Nephropoidea; Nephropidae; Homarus. REFERENCE 1 (bases 1 to 629) AUTHORS Towle,D.W., Smith,C.M. and Beale,K.M. TITLE Expressed Sequence Tags in a normalized cDNA library prepared from multiple tissues of adult intermolt and postmolt American lobster, Homarus americanus JOURNAL Unpublished COMMENT Contact: Christine M. Smith Marine DNA Sequencing and Analysis Facility Mount Desert Island Biological Laboratory Old Bar Harbor Road, Salsbury Cove, ME 04672, USA Tel: 207-288-9880 x130 Fax: 207-288-2130 Email: cmsmith@mdibl.org Plate: 35 row: e column: 10 Seq primer: SP6. FEATURES Location/Qualifiers source 1..629 /organism="Homarus americanus" /mol_type="mRNA" /db_xref="taxon:6706" /clone="HA_MX2_35e10" /tissue_type="Gill, epipodite, branchiostegite, heart, ovary, testis, antennal gland, abdominal muscle, hepatopancreas, brain" /clone_lib="SAMN00152457 Lobster Multiple Tissues, Normalized" /dev_stage="Adult intermolt and postmolt" /note="Vector: pCMV Sport 6.1; Total RNA samples were prepared individually from each tissue, checked for quality, then pooled for construction and normalization of cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at Mount Desert Island Biological Laboratory. Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software)." BASE COUNT 109 a 198 c 153 g 169 t ORIGIN 1 atcatgtgtg agatatgagg aactctctct ctctctatct tattgtggcc actacacctg 61 ctgtggccac tacacctgct gtggccgata cacctgctgt ggccgataca cctgctgtgg 121 ccgatacacc tgctgtggcc gatacacctg ctgtggccac tacacctgct gtggccacta 181 cacctgctgt ggccactaca cctgctgtag ccactacagc tcttgtggcc gatacacctg 241 ctgtggccac taaacctgct gtggccatta cacctgttgt ggccacatct tttgtggcca 301 ctacacctgc tgtggacact acacctattg tgcccactac ttctggtgtg cccactacag 361 ctcttgtggc cgttacagct cttgtggccg ttacagctct tgtgggcact acagctcttg 421 tgggcactac agctcttgtg ggcactacag ctcttgtggg cactacagct cttgtgggca 481 ctacagctct tgtgggcact acagctcttg tgggcactac agctcttgtg ggcactacag 541 ctctgcggcc actacagctc ttgcggccgc tctagagtat ccctcgaggg gcccaagctt 601 acgcgtaccc agctttcttg tacaagtgg //