LOCUS FD483315 724 bp mRNA linear EST 30-JUN-2009 DEFINITION HA_MX2_35e04_SP6 Lobster Multiple Tissues, Normalized Homarus americanus cDNA clone HA_MX2_35e04 5', mRNA sequence. ACCESSION FD483315 VERSION FD483315.1 DBLINK BioSample: SAMN00152457 KEYWORDS EST. SOURCE Homarus americanus (American lobster) ORGANISM Homarus americanus Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Astacidea; Nephropoidea; Nephropidae; Homarus. REFERENCE 1 (bases 1 to 724) AUTHORS Towle,D.W., Smith,C.M. and Beale,K.M. TITLE Expressed Sequence Tags in a normalized cDNA library prepared from multiple tissues of adult intermolt and postmolt American lobster, Homarus americanus JOURNAL Unpublished COMMENT Contact: Christine M. Smith Marine DNA Sequencing and Analysis Facility Mount Desert Island Biological Laboratory Old Bar Harbor Road, Salsbury Cove, ME 04672, USA Tel: 207-288-9880 x130 Fax: 207-288-2130 Email: cmsmith@mdibl.org Plate: 35 row: e column: 04 Seq primer: SP6 High quality sequence start: 2 High quality sequence stop: 723. FEATURES Location/Qualifiers source 1..724 /organism="Homarus americanus" /mol_type="mRNA" /db_xref="taxon:6706" /clone="HA_MX2_35e04" /tissue_type="Gill, epipodite, branchiostegite, heart, ovary, testis, antennal gland, abdominal muscle, hepatopancreas, brain" /clone_lib="SAMN00152457 Lobster Multiple Tissues, Normalized" /dev_stage="Adult intermolt and postmolt" /note="Vector: pCMV Sport 6.1; Total RNA samples were prepared individually from each tissue, checked for quality, then pooled for construction and normalization of cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at Mount Desert Island Biological Laboratory. Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software)." BASE COUNT 211 a 110 c 134 g 269 t ORIGIN 1 gctggtaccg gtccggaatt ccgggatctg ttatctattg ttgggaacta cattggtact 61 tgtttaagtg gtattatacg tatacagtac aattttaatg tggaaaagtt gagtctgcat 121 atactgtatt ttgtttatgt tgcattatcc aacttactga acaagaatca gactgtctga 181 ctaactgaca tgaccaactg acttactgat ggaggcaact tttagcccat cttacacttc 241 tgtagcttat aacttttcta tttttattta tcatgcttta tttttaccat atcctgtgca 301 tattaagtaa tttcccttaa agtcacctct gttagggata ggaggctcat catgaacata 361 cctgagtgag tttatggtag attcttgtta tttaggcact agaaggttac attacttata 421 gagcatttaa acaagattat agtcttaagt gtacatgatg tattaggcag tttcagagac 481 taaaaatgtt aaaagggtga atatgctgtg tttaatgtgg aaaattgaca gcttaatttt 541 tttattcagt gtacagttta tttatttgtt ttattttcat actgtttggg ttaatggagg 601 agtgagttca ggaatcatca gaagtgcaga ctatcagata atgacagtgt aaacaacatt 661 taattcatat attatctagt aagatgttcc cacttactca taggacatct gataaccttc 721 cctt //