LOCUS FD483311 663 bp mRNA linear EST 30-JUN-2009
DEFINITION HA_MX2_35d09_SP6 Lobster Multiple Tissues, Normalized Homarus
americanus cDNA clone HA_MX2_35d09 5', mRNA sequence.
ACCESSION FD483311
VERSION FD483311.1
DBLINK BioSample: SAMN00152457
KEYWORDS EST.
SOURCE Homarus americanus (American lobster)
ORGANISM Homarus americanus
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea;
Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda;
Pleocyemata; Astacidea; Nephropoidea; Nephropidae; Homarus.
REFERENCE 1 (bases 1 to 663)
AUTHORS Towle,D.W., Smith,C.M. and Beale,K.M.
TITLE Expressed Sequence Tags in a normalized cDNA library prepared from
multiple tissues of adult intermolt and postmolt American lobster,
Homarus americanus
JOURNAL Unpublished
COMMENT Contact: Christine M. Smith
Marine DNA Sequencing and Analysis Facility
Mount Desert Island Biological Laboratory
Old Bar Harbor Road, Salsbury Cove, ME 04672, USA
Tel: 207-288-9880 x130
Fax: 207-288-2130
Email: cmsmith@mdibl.org
Plate: 35 row: d column: 09
Seq primer: SP6
High quality sequence stop: 662.
FEATURES Location/Qualifiers
source 1..663
/organism="Homarus americanus"
/mol_type="mRNA"
/db_xref="taxon:6706"
/clone="HA_MX2_35d09"
/tissue_type="Gill, epipodite, branchiostegite, heart,
ovary, testis, antennal gland, abdominal muscle,
hepatopancreas, brain"
/clone_lib="SAMN00152457 Lobster Multiple Tissues,
Normalized"
/dev_stage="Adult intermolt and postmolt"
/note="Vector: pCMV Sport 6.1; Total RNA samples were
prepared individually from each tissue, checked for
quality, then pooled for construction and normalization of
cDNA library by Invitrogen. Plasmids were isolated and
inserts end-sequenced by the Marine DNA Sequencing and
Analysis Facility at Mount Desert Island Biological
Laboratory. Traces were processed for submission to dbEST
by trace2dbest software (Parkinson, Anthony and Blaxter,
unpublished software)."
BASE COUNT 178 a 192 c 112 g 181 t
ORIGIN
1 atcggtttac ctctctgtag gctacccaat cttacagcat agctatcttt gtccacaaca
61 agcagcgcac ctacccattc aaacctaaca agatagtaag cgatgtcttc ttcacgataa
121 acaggcaaac taggttttac attatcttgt ctagggccag aggattctgg tctactttga
181 gcattaatcc tagctggttc tatgtccttc tctgcctgca acttagctaa ttcgaactct
241 ttctctgttg cattcctctc tctctcgctg ttgcattcct ctctctctcg ctattgcttt
301 catctttttg ttgcgttgct ctctctcggc agtctgttgg gagatcacat actgaccaac
361 ctccgcacct gtcagaccca gggccaccgc ttcacctttc aactcctcaa aactagacat
421 aatgatacaa aaggccaaga gccaaagtga caaattcaac aacttacccg gcaactagcc
481 ataggtaact acttagtggc tcaatcaaac cattaaatga ttcgatcccg ctggaggaaa
541 ccttgtttag cacccagtcc agttgaatca cctcgggaaa agaaccgcac caggtgaatc
601 aacaacaaat gctatggcag cctccacagt gcaccccgaa caacactaaa actccctagt
661 atg
//