LOCUS FD483305 711 bp mRNA linear EST 30-JUN-2009 DEFINITION HA_MX2_35d03_SP6 Lobster Multiple Tissues, Normalized Homarus americanus cDNA clone HA_MX2_35d03 5' similar to ref|XP_001605900.1| PREDICTED: similar to ENSANGP00000018727 - Nasonia vitripennis. Score = 298 bits (763), Expect = 2e-79, mRNA sequence. ACCESSION FD483305 VERSION FD483305.1 DBLINK BioSample: SAMN00152457 KEYWORDS EST. SOURCE Homarus americanus (American lobster) ORGANISM Homarus americanus Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Astacidea; Nephropoidea; Nephropidae; Homarus. REFERENCE 1 (bases 1 to 711) AUTHORS Towle,D.W., Smith,C.M. and Beale,K.M. TITLE Expressed Sequence Tags in a normalized cDNA library prepared from multiple tissues of adult intermolt and postmolt American lobster, Homarus americanus JOURNAL Unpublished COMMENT Contact: Christine M. Smith Marine DNA Sequencing and Analysis Facility Mount Desert Island Biological Laboratory Old Bar Harbor Road, Salsbury Cove, ME 04672, USA Tel: 207-288-9880 x130 Fax: 207-288-2130 Email: cmsmith@mdibl.org Plate: 35 row: d column: 03 Seq primer: SP6 High quality sequence start: 2 High quality sequence stop: 710. FEATURES Location/Qualifiers source 1..711 /organism="Homarus americanus" /mol_type="mRNA" /db_xref="taxon:6706" /clone="HA_MX2_35d03" /tissue_type="Gill, epipodite, branchiostegite, heart, ovary, testis, antennal gland, abdominal muscle, hepatopancreas, brain" /clone_lib="SAMN00152457 Lobster Multiple Tissues, Normalized" /dev_stage="Adult intermolt and postmolt" /note="Vector: pCMV Sport 6.1; Total RNA samples were prepared individually from each tissue, checked for quality, then pooled for construction and normalization of cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at Mount Desert Island Biological Laboratory. Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software)." BASE COUNT 172 a 171 c 184 g 184 t ORIGIN 1 ccgggatagg tgatagtgaa ggctcgctgc tcctctacgg caggttattt gattgtaggt 61 gaatggtgga gtgggcgcgc tggatatggt aagccggata gatcagatct atcgtagcat 121 tctcctgaca aagttctttg ttcgggggtg gggcaaacct gaaaatctcc gcagactttt 181 tgccttccga aagatcctct ccaaccgtga aacatgccag caacttgtcg acaaaaacca 241 tccaattact ataactaagg aagttgacaa tggagatcac atagttttgg aagcagagtt 301 catctcccca tttgtggctc acctgccggg tcttctgccc aaggaatcgg agaaagccac 361 cttccagttg gtgtttccta aagtatggcg gactcacctc aagcccacct gtctccacct 421 agctggcaca ggagatcacg gattttggag aagaaaaatg cttatgggga aaccacttct 481 ggacgagtca ggtatcgctt cagctatatt agagaatccc ttttatgggt gccggaaacc 541 caaggaccaa atccgctcaa gcttacacaa cgtttcggac ttatttgtga tgggcggttg 601 tctcgttctg gagtctctcg ccatattcaa ctggtgtgaa cgcctgggct ttggacctct 661 gggaatcact gggatctcca tgggcgggca catggcatct ttggcagcta c //