LOCUS FD483295 674 bp mRNA linear EST 30-JUN-2009 DEFINITION HA_MX2_35b12_SP6 Lobster Multiple Tissues, Normalized Homarus americanus cDNA clone HA_MX2_35b12 5', mRNA sequence. ACCESSION FD483295 VERSION FD483295.1 DBLINK BioSample: SAMN00152457 KEYWORDS EST. SOURCE Homarus americanus (American lobster) ORGANISM Homarus americanus Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Astacidea; Nephropoidea; Nephropidae; Homarus. REFERENCE 1 (bases 1 to 674) AUTHORS Towle,D.W., Smith,C.M. and Beale,K.M. TITLE Expressed Sequence Tags in a normalized cDNA library prepared from multiple tissues of adult intermolt and postmolt American lobster, Homarus americanus JOURNAL Unpublished COMMENT Contact: Christine M. Smith Marine DNA Sequencing and Analysis Facility Mount Desert Island Biological Laboratory Old Bar Harbor Road, Salsbury Cove, ME 04672, USA Tel: 207-288-9880 x130 Fax: 207-288-2130 Email: cmsmith@mdibl.org Plate: 35 row: b column: 12 Seq primer: SP6 High quality sequence stop: 673. FEATURES Location/Qualifiers source 1..674 /organism="Homarus americanus" /mol_type="mRNA" /db_xref="taxon:6706" /clone="HA_MX2_35b12" /tissue_type="Gill, epipodite, branchiostegite, heart, ovary, testis, antennal gland, abdominal muscle, hepatopancreas, brain" /clone_lib="SAMN00152457 Lobster Multiple Tissues, Normalized" /dev_stage="Adult intermolt and postmolt" /note="Vector: pCMV Sport 6.1; Total RNA samples were prepared individually from each tissue, checked for quality, then pooled for construction and normalization of cDNA library by Invitrogen. Plasmids were isolated and inserts end-sequenced by the Marine DNA Sequencing and Analysis Facility at Mount Desert Island Biological Laboratory. Traces were processed for submission to dbEST by trace2dbest software (Parkinson, Anthony and Blaxter, unpublished software)." BASE COUNT 179 a 154 c 152 g 188 t ORIGIN 1 cgaacaactc agtttttaac cttattaaca atgtaaagat tttccttttg ccagacatga 61 taaggtggat gtgtgagtgt aatgaccgtg atcgttgcac tgtgaatcat ctgattttac 121 gttaactaac aagtacgacc gtgggctctg ggacttggtc gcataggcag cagggtctac 181 cattaaacaa cacattgtgc ttccattatc ttcccgggca gtagtacaca ccactgtaat 241 gtcataacct ttagctctgc cccattcaat gacaccacgc tacctgtggt gaccacaagg 301 actacggtct tgccttgttc acgtttgact gtttcatttt ttttaagaac actcgtaatg 361 caaaggcttt tctttggcca ggtataacaa aatgaatgcc ttatgttctg cttatactac 421 agacgggcaa gggacgacct aattgaggta tataagcatc tcaaggggca ctactcagtc 481 gagtgtcctt acctggagct ggcagatgct cgccccacaa ggggtcacag ctcaatgttg 541 aagaagccgc aggtccagaa aactgtaagg ngtaactttt tcggagtgag agttgtaaac 601 tcttgcaaca atctgccaga gagtgttgtg acggcaccga gtgtgagctc tttcagacca 661 gacttgacca cact //