LOCUS FC456641 443 bp mRNA linear EST 13-NOV-2008 DEFINITION Ssh-drought39 Soybean nodule 5 days after drought library Glycine max cDNA similar to unknown function protein homologue to cupin RmC-type protein, mRNA sequence. ACCESSION FC456641 VERSION FC456641.1 DBLINK BioSample: SAMN00164565 KEYWORDS EST. SOURCE Glycine max (soybean) ORGANISM Glycine max Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Glycine subgen. Soja. REFERENCE 1 (bases 1 to 443) AUTHORS Clement,M., Lambert,A., Herouart,D. and Bomcompagni,D. TITLE Identification of new up-regulated genes under drought stress in soybean nodules JOURNAL Gene 426 (1-2), 15-22 (2008) PUBMED 18817859 COMMENT Contact: Boncompagni E UMR Interactions Plantes Microrganismes INRA (1064) CNRS (6192) Universite de Nice Sophia Antipolis 400 route des Chappes, BP 167, Sophia antipolis cedex, F-06903, FRANCE Tel: 00 33 4 92 38 66 34 Fax: 00 33 4 92 38 66 40 Email: boncompa@unice.fr Insert Length: 443 Std Error: 0.00 Seq primer: T7. FEATURES Location/Qualifiers source 1..443 /organism="Glycine max" /mol_type="mRNA" /cultivar="Labrador" /db_xref="taxon:3847" /clone_lib="SAMN00164565 Soybean nodule 5 days after drought library" /dev_stage="mature soybean, six weeks old" /note="Organ: nodule; SSH method was used to isolate gene specifically up-regulated in response to drought in soybean nodule. mRNA were extracted from control plant and from drought stressed (5 days without watering) plant. cDNA obtained after subtraction were cloned into pGEMT vector and sequenced with the T7 primer." BASE COUNT 124 a 106 c 97 g 116 t ORIGIN 1 caggtacggt tttcagtaag ctactttttg gcactatgca catcaagtca tatgattggg 61 tcgttgactt gcctcctgaa tctcctacaa ctatcaaacc atcagaaaat caaggtcctg 121 agatgaggtt ggccaaagtt aaggttgatg ctgatttcac tgctccctgc aacccctcca 181 tcctttatcc agaagatggg ggcaacttgc actgtttcac agcagtgaca gcatgcgcag 241 ttctagatgt gctcggtcct ccatattctg atgctgaagg cagacactgc acatactacc 301 acgatttccc cttttccaac ttttcagtgg atggattatc cataccagaa gaggaaaaga 361 atgcttatga atggctacaa gagagggatg aacttgaaga cctagaagta aatggaaaaa 421 tgtacctcgg ccgcgaccac gct //