LOCUS FC456620 1042 bp mRNA linear EST 13-NOV-2008 DEFINITION Ssh-drought16 Soybean nodule 5 days after drought library Glycine max cDNA similar to unknown function protein homologue to cytosolic phosphoglycerate kinase, mRNA sequence. ACCESSION FC456620 VERSION FC456620.1 DBLINK BioSample: SAMN00164565 KEYWORDS EST. SOURCE Glycine max (soybean) ORGANISM Glycine max Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Glycine subgen. Soja. REFERENCE 1 (bases 1 to 1042) AUTHORS Clement,M., Lambert,A., Herouart,D. and Bomcompagni,D. TITLE Identification of new up-regulated genes under drought stress in soybean nodules JOURNAL Gene 426 (1-2), 15-22 (2008) PUBMED 18817859 COMMENT Contact: Boncompagni E UMR Interactions Plantes Microrganismes INRA (1064) CNRS (6192) Universite de Nice Sophia Antipolis 400 route des Chappes, BP 167, Sophia antipolis cedex, F-06903, FRANCE Tel: 00 33 4 92 38 66 34 Fax: 00 33 4 92 38 66 40 Email: boncompa@unice.fr Insert Length: 1043 Std Error: 0.00 Seq primer: T7. FEATURES Location/Qualifiers source 1..1042 /organism="Glycine max" /mol_type="mRNA" /cultivar="Labrador" /db_xref="taxon:3847" /clone_lib="SAMN00164565 Soybean nodule 5 days after drought library" /dev_stage="mature soybean, six weeks old" /note="Organ: nodule; SSH method was used to isolate gene specifically up-regulated in response to drought in soybean nodule. mRNA were extracted from control plant and from drought stressed (5 days without watering) plant. cDNA obtained after subtraction were cloned into pGEMT vector and sequenced with the T7 primer." BASE COUNT 261 a 196 c 266 g 279 t ORIGIN 1 gaggtcgtcc aaaaggtgta acacctaaat acagtttgaa gcctcttgtg ccaaggctgt 61 ttcaacttct tggaattgag gttacgatgg ccaatgactc tattggggag gaagttgaaa 121 agttggttac acaacttcca gaaggtggtg ttttgcttct agagaatgtg aagttctaca 181 aggaggaaga gaagaatgat cctgaatttg caaagaagtt ggcttctcta gctgatctct 241 atgtgaatga tgcatttggc actgcccaca gagctcatgc ttctacagaa ggagttgcca 301 agtacttgaa gccctctgtt gcaggattcc taatgcagaa ggagcttgat tatctagttg 361 gggctgtgtc aaaccccaag agaccatttg ctgctattgt cggtggatcg aaggtgtctt 421 ccaagattgg agttattgaa tccttgttgg agaaagttaa tgttctcttg cttggtggag 481 gaatgatctt taccttttac aaggctcagg gttattcagt tgggtcatct cttgtggagg 541 aagacaagct tagccttgca actacacttc ttgaaaaggc caaggctaaa ggggtttctt 601 tgttgcttcc aactgatgtt gtcatagcag acaagtttgc tgctgatgct aacagcaaga 661 ctgtgccggc atcaagcatc ccagatgggt ggatggggtt ggatattggg tcctgattcc 721 atcaagacat tttggtgaag cattggatac gactcanacc actatttgga atggaccaat 781 gggtgttttt gagtttgata agtttgccnc aggaacagag gctatagcca agaaactggc 841 anagctgant gggaagggag tgacaaccat cattgggagg aggtgactcc cttgcccctg 901 tggaaaangt tggnacttnc aaacnanatg anccncntct ccccctgggn ggnggngncc 961 nncntnaaan ctnctttnag ggnaaancaa ccccccnggg gnccntgccc nttganaann 1021 cttgnnnnaa aaannnttcn tt //