LOCUS FC125450 496 bp mRNA linear EST 06-DEC-2007 DEFINITION 1106878631167 BABEVPN-C-01-1-7KB Papio anubis cDNA clone 1061041584396 3', mRNA sequence. ACCESSION FC125450 VERSION FC125450.1 DBLINK BioSample: SAMN00164535 KEYWORDS EST. SOURCE Papio anubis (olive baboon) ORGANISM Papio anubis Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Papio. REFERENCE 1 (bases 1 to 496) AUTHORS Drees,R.L., Chu,V.T., Sova,P., Lehvaslaiho,H., Jonas,M., Lehvaslaiho,H., Hide,W., Straussberg,R.L. and Bumgarner,R.E. TITLE Evolutionary insights from large scale Papio anubis cDNA sequencing project JOURNAL Unpublished COMMENT Other_ESTs: 1106800402205 Contact: Bumgarner R Bumgarner Lab, Department of Microbiology University of Washington UW, Box 358070, 960 Republican Ave, Seattle, WA 98104, USA Tel: 206-732-6137 Fax: 206-732-6055 Email: rogerb@u.washington.edu Institutes contributing to this submission are: University of Washington (UW), Seattle, USA; South African National Bioinformatics Institute (SANBI), University of the Western Cape, South Africa and J. Craig Venter Institute (JCVI), Rockville, USA. Tissue samples were provided by Southwest Foundation for Biomedical Research (SFBR), San Antonio, USA. Additional details are available at ftp://baboon.washington.edu/. Plate: BB0211725AB row: C column: 4 Seq primer: CCAGGGTTTTCCCAGTCACG. FEATURES Location/Qualifiers source 1..496 /organism="Papio anubis" /mol_type="mRNA" /db_xref="taxon:9555" /clone="1061041584396" /sex="female" /clone_lib="SAMN00164535 BABEVPN-C-01-1-7KB" /note="Organ: placenta; Vector: pAL17.3; Site_1: Sfi1A; Site_2: Sfi1B; Poly A RNA was prepared from total RNA prep. Full-size, double-stranded cDNA was prepared by SMART synthesis and normalized using kamchatka crab duplex-specific nuclease. SfiI-digested cDNA was cloned in SfiIA and SfiIB sites in pAL17.3 plasmid vector. Sequencing was carried out on a template consisting of vector DNA from cDNA-carrying clones selected by blue/white screeing." BASE COUNT 143 a 99 c 121 g 133 t ORIGIN 1 accggaagtc gagcctccgc tgtgggcctg cgagaatcga ggcactcgcc ggcgtaccca 61 tgtatagaag agagttcacg gcctggtacc ggcggatgtc ggtggtctac gggatcggcg 121 cctggtctgt gttgggctca ctgttttact acaactggac aatggcgaag tcatcagacc 181 gaaaggatgg cttaacaact gaaacaccag gtgaagtatc tgaacaccca aaaggatttt 241 atgtggaaac agttatcata tataaagaag attttgttcc aattacagaa aagatcctca 301 actattggaa atcgtggact ggtggccctg gtgcagaacc atgactggct gccgaattct 361 gaaaaccagg acttggttca acatttaaat ttgatagttg cctcgattcc cattttgtgt 421 ttgtgaaagg tgtatgtatt taaaattgct ataaaacata atcactaata atctgcaata 481 aatattttct tgaagg //