LOCUS FC125422 177 bp mRNA linear EST 06-DEC-2007 DEFINITION 1106878628731 BABEVPN-C-01-1-7KB Papio anubis cDNA clone 1061041580028 5', mRNA sequence. ACCESSION FC125422 VERSION FC125422.1 DBLINK BioSample: SAMN00164535 KEYWORDS EST. SOURCE Papio anubis (olive baboon) ORGANISM Papio anubis Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Papio. REFERENCE 1 (bases 1 to 177) AUTHORS Drees,R.L., Chu,V.T., Sova,P., Lehvaslaiho,H., Jonas,M., Lehvaslaiho,H., Hide,W., Straussberg,R.L. and Bumgarner,R.E. TITLE Evolutionary insights from large scale Papio anubis cDNA sequencing project JOURNAL Unpublished COMMENT Other_ESTs: 1106878571323 Contact: Bumgarner R Bumgarner Lab, Department of Microbiology University of Washington UW, Box 358070, 960 Republican Ave, Seattle, WA 98104, USA Tel: 206-732-6137 Fax: 206-732-6055 Email: rogerb@u.washington.edu Institutes contributing to this submission are: University of Washington (UW), Seattle, USA; South African National Bioinformatics Institute (SANBI), University of the Western Cape, South Africa and J. Craig Venter Institute (JCVI), Rockville, USA. Tissue samples were provided by Southwest Foundation for Biomedical Research (SFBR), San Antonio, USA. Additional details are available at ftp://baboon.washington.edu/. Plate: BB0211808AB row: M column: 4 Seq primer: TTTTTTTTTTTTTTTTTV. FEATURES Location/Qualifiers source 1..177 /organism="Papio anubis" /mol_type="mRNA" /db_xref="taxon:9555" /clone="1061041580028" /sex="female" /clone_lib="SAMN00164535 BABEVPN-C-01-1-7KB" /note="Organ: placenta; Vector: pAL17.3; Site_1: Sfi1A; Site_2: Sfi1B; Poly A RNA was prepared from total RNA prep. Full-size, double-stranded cDNA was prepared by SMART synthesis and normalized using kamchatka crab duplex-specific nuclease. SfiI-digested cDNA was cloned in SfiIA and SfiIB sites in pAL17.3 plasmid vector. Sequencing was carried out on a template consisting of vector DNA from cDNA-carrying clones selected by blue/white screeing." BASE COUNT 63 a 34 c 26 g 54 t ORIGIN 1 aaatccaatt atacaaaaaa tactacatct agtctggggt agatatattt atttttggta 61 acatacatta agtggcacta attacacagt aactataagg taactaacat gaaaccacaa 121 aactgtaact ctgccacaac tgcatgaact tgggcttttc tggttgagcc cattttc //