LOCUS FC125220 335 bp mRNA linear EST 06-DEC-2007 DEFINITION 1106878627898 BABEVPN-C-01-1-7KB Papio anubis cDNA clone 1061041587050 5' similar to H. sapiens MRPL27 (UniProtKB/Swiss-Prot:Q9P0M9), mRNA sequence. ACCESSION FC125220 VERSION FC125220.1 DBLINK BioSample: SAMN00164535 KEYWORDS EST. SOURCE Papio anubis (olive baboon) ORGANISM Papio anubis Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Papio. REFERENCE 1 (bases 1 to 335) AUTHORS Drees,R.L., Chu,V.T., Sova,P., Lehvaslaiho,H., Jonas,M., Lehvaslaiho,H., Hide,W., Straussberg,R.L. and Bumgarner,R.E. TITLE Evolutionary insights from large scale Papio anubis cDNA sequencing project JOURNAL Unpublished COMMENT Other_ESTs: 1106878576250 Contact: Bumgarner R Bumgarner Lab, Department of Microbiology University of Washington UW, Box 358070, 960 Republican Ave, Seattle, WA 98104, USA Tel: 206-732-6137 Fax: 206-732-6055 Email: rogerb@u.washington.edu Institutes contributing to this submission are: University of Washington (UW), Seattle, USA; South African National Bioinformatics Institute (SANBI), University of the Western Cape, South Africa and J. Craig Venter Institute (JCVI), Rockville, USA. Tissue samples were provided by Southwest Foundation for Biomedical Research (SFBR), San Antonio, USA. Additional details are available at ftp://baboon.washington.edu/. Plate: BB0203866AB row: A column: 18 Seq primer: TTTTTTTTTTTTTTTTTV. FEATURES Location/Qualifiers source 1..335 /organism="Papio anubis" /mol_type="mRNA" /db_xref="taxon:9555" /clone="1061041587050" /sex="female" /clone_lib="SAMN00164535 BABEVPN-C-01-1-7KB" /note="Organ: placenta; Vector: pAL17.3; Site_1: Sfi1A; Site_2: Sfi1B; Poly A RNA was prepared from total RNA prep. Full-size, double-stranded cDNA was prepared by SMART synthesis and normalized using kamchatka crab duplex-specific nuclease. SfiI-digested cDNA was cloned in SfiIA and SfiIB sites in pAL17.3 plasmid vector. Sequencing was carried out on a template consisting of vector DNA from cDNA-carrying clones selected by blue/white screeing." BASE COUNT 72 a 105 c 79 g 79 t ORIGIN 1 agtctcctgc agagtcacca tcaagcagac ctcttccttg ggaggccgtg tcgtcgcccc 61 aacccttgac ttctggtatc accatctcct gtcacctggg ccccagtctc tgtctgatgg 121 cctcaacagg acatcaaagc atagctacca gtttgaaggt gccctccggc ttggcaggaa 181 ccacgtggac aaaagtcttg tagagtacag cacccttggg cagcctggtg atcagatcca 241 cagcctccgt gcttctggga tgaggcacgt agacctcctt agtgtagcgg actatcccct 301 cttccagggc atacagacat ttattcttcc caaga //