LOCUS EY195632 559 bp mRNA linear EST 08-NOV-2007
DEFINITION RSAA-aab24e05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
similar to ref|NP_492721.1| Putative membrane protein, with at
least 2 transmembrane domains, of eukaryotic origin (28.5 kD)
[Caenorhabditis elegans] pir|T21337 hypothetical protein F25D7.1 -
Caenorhabditis elegans emb|CAB01696.1| Hypothetical protein F25D7,
mRNA sequence.
ACCESSION EY195632
VERSION EY195632.1
DBLINK BioSample: SAMN00153695
KEYWORDS EST.
SOURCE Radopholus similis
ORGANISM Radopholus similis
Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
Tylenchina; Tylenchomorpha; Tylenchoidea; Pratylenchidae;
Radopholinae; Radopholus.
REFERENCE 1 (bases 1 to 559)
AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
Scott,A.L., McCarter,J.P. and Wilson,R.
TITLE WashU Nematode EST Project
JOURNAL Unpublished
COMMENT Contact: Dr. Makedonka Mitreva
WashU Nematode EST Project
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
Tel: 314 286 1800
Fax: 314 286 1810
Email: submissions@watson.wustl.edu
Library material provided by Dr. Godelieve Gheysen Library
constructed by Dept. of Molecular Biotechnology,Ghent University
Library sequenced by Washington University Genome Sequencing Center
This trace has been recalled with phred
original value before phred recall for SL was 148
original value before phred recall for SR was 840
Seq primer: -21UPpOT.
FEATURES Location/Qualifiers
source 1..559
/organism="Radopholus similis"
/mol_type="mRNA"
/db_xref="taxon:46012"
/clone_lib="SAMN00153695 R.similis_EST_RSAA"
/dev_stage="mixed"
/lab_host="DH5alpha"
/note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
BASE COUNT 126 a 117 c 114 g 202 t
ORIGIN
1 ggccggggat agataatatc tcaggcactt actttttccc gtcatttttt aagaaatttt
61 tttctctctc ctactactag gactcttaaa atttgattgg cttttgggaa gtacgtccgt
121 ttctatctaa attaaaattt tagtccctat atgatgttac tcaactggga actgttcttc
181 tacaaatttc aaatttggcg tccgctgact gctctattct actacccagt tacgcctcag
241 accggattcc attggctgtt gatgtgctac tttctctaca actattcgaa agaattagag
301 atatcttttt tcgatgggag gccagccgat tattttttca tgctcatctt taactggctc
361 tgttgcacag tgatctgctt tgtggcagaa atttattttc tgctcgaacc gatggtgtta
421 agtgttctct acgtttggtg ccaattcaac aaggaccgca ccgttcagtt ttggtttgga
481 atgcagttcg aggccgtcta tttgccctgg gtgttggtcg ctttcaacat gatattgcgc
541 ggtggaagca taaatgaac
//