LOCUS EX016247 479 bp mRNA linear EST 11-MAR-2011
DEFINITION BR000891 anther cDNA library KBAY Brassica rapa subsp. pekinensis
cDNA clone KBAY-15F10 5', mRNA sequence.
ACCESSION EX016247
VERSION EX016247.1
DBLINK BioSample: SAMN00153461
KEYWORDS EST.
SOURCE Brassica rapa subsp. pekinensis (Brassica campestris (Pekinensis
Group))
ORGANISM Brassica rapa subsp. pekinensis
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
Brassiceae; Brassica.
REFERENCE 1 (bases 1 to 479)
AUTHORS Lee,S.C., Lim,M.H., Kim,J.A., Lee,S.I., Kim,J.S., Jin,M.,
Kwon,S.J., Mun,J.H., Kim,Y.K., Kim,H.U., Hur,Y. and Park,B.S.
TITLE Transcriptome analysis in Brassica rapa under the abiotic stresses
using Brassica 24K oligo microarray
JOURNAL Mol. Cells 26 (6), 595-605 (2008)
PUBMED 18797175
COMMENT Contact: NIAB
Brassica Genomics Team; National Agricutural Biotchnology
Information Center (NABIC)
National Institute of Agricultural Biotechnology (NIAB
http://www.niab.go.kr/homepage/english/), Rural Development
Administration (RDA http://www3.rda.go.kr/eng/)
252 Seodoon-dong, Kwonsun-ku, Suwon, 441-707 K0REA
Tel: 82 31 299 1615
Fax: 82 31 299 1664
Email: niab@rda.go.kr
This work was supported by National Institute of Agricultural
Biotechnology (Project 04-1-12-2-3, Lim, M.-H., NIAB) and
BioGreen21 Program (Project 20050301034448, Hur, Y., Chungnam
National Univ) of Rural Development Administration in Korea.
Sequences have been trimmed to remove vector sequences and low
quality regions.
Seq primer: T3.
FEATURES Location/Qualifiers
source 1..479
/organism="Brassica rapa subsp. pekinensis"
/mol_type="mRNA"
/cultivar="Chiifu"
/sub_species="pekinensis"
/db_xref="taxon:51351"
/clone="KBAY-15F10"
/clone_lib="SAMN00153461 anther cDNA library KBAY"
/dev_stage="immature anthers in floral bud"
/note="Organ: anther; Vector: pDNR-LIB; Site_1: Sfi IA;
Site_2: Sfi IB; Immature anthers isolated from floral buds
of B. rapa plant were used for the total RNA extraction.
The cDNA library was constructed using CreatorTM SMARTTM
cDNA library construction kit (Clonetech, USA) according
to the manufacturer's instructions and randomly selected
cDNA clones were used for the 5-end sequence analysis."
BASE COUNT 135 a 105 c 123 g 116 t
ORIGIN
1 ggagaaagcg gtggctatgg agaagaaggt ggctatggaa gtggctatgg agaaggtagt
61 ggctcaggcg aagggattgg tatcggcgta ggaggtgact ctagtggtag tgtaccaagt
121 gtagttgtcc caagcatatc aatccctccc ataacggttc caggcgcaca gattcccggt
181 tttatgattc caggggtcag ggttaatcct ggttatggtt ctgggggaag ccaaaccggt
241 ggttgcaccc caagcgtacc atactaccgc ccacctatat accaacaacc accaagctgt
301 tcccattgtg cacctttcgt gtcaggacaa gacaagcaca tgtcagacaa aggaactatg
361 accgaagaag ctcttgcacc cacttcaaac gggatgcttg tctaagcttt cagtgtccat
421 agaaacacaa aagtataata aaatcactca taaggatata tatatcgtat tttggtttt
//