LOCUS EU912542 7079 bp DNA circular SYN 26-JUL-2016 DEFINITION Expression vector pDM358, complete sequence. ACCESSION EU912542 VERSION EU912542.1 KEYWORDS . SOURCE Expression vector pDM358 ORGANISM Expression vector pDM358 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7079) AUTHORS Veltman,D.M., Akar,G., Bosgraaf,L. and Van Haastert,P.J. TITLE A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum JOURNAL Plasmid 61 (2), 110-118 (2009) PUBMED 19063918 REFERENCE 2 (bases 1 to 7079) AUTHORS Veltman,D.M., Akar,G., Bosgraaf,L. and van Haastert,P.J.M. TITLE Direct Submission JOURNAL Submitted (19-JUL-2008) Cell Biochemistry, Rijks Universiteit Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands FEATURES Location/Qualifiers source 1..7079 /organism="Expression vector pDM358" /mol_type="other DNA" /db_xref="taxon:555776" /note="modular expression vector (hyg)" regulatory 14..303 /regulatory_class="promoter" /note="act15 promoter" regulatory 332..594 /regulatory_class="terminator" /note="act8 terminator" CDS <606..1091 /codon_start=1 /transl_table=11 /product="D1'" /protein_id="ACH81561.1" /translation="FEKVNSNYYKIQSESTQINSNEIADLKKFIKEEVNKTSSKIDFF LVSSTDALSNPENYSLLEVKCINCHSLCQGKNLYISCTRDGCQNNICYNCLGININIY NVVINSKLCPPCFNDSVINKKCAMCSKNGTKCNLNQECKLHLCAQCSKKCLYILRVKT N" CDS 1594..2358 /codon_start=1 /transl_table=11 /product="D5" /protein_id="ACH81562.1" /translation="MYSTTLLFFLFFFIVSKSSVLQNDQIEVKYFQLLIVMYQKENCV EKSMTGAVIYDECNIHGRVETNSTHALFYDDIETNNSRCNNFRNLTNLIKLNECINDE FGESILYKEYNETDDGYLFRVEDSFVEITSLSMDCTKNSKTIIEKFNICSKFENVYHI TNITQEKSNRFTCTDPLCHYCKNENIQNNLDFKTTKCTPKYGASDSEFLSTIYNPKLD GSNNGMEKSVTQEKNISNNLKINIYLIFFLIIFLIK" CDS complement(2366..2959) /codon_start=1 /transl_table=11 /product="G5" /protein_id="ACH81563.1" /translation="MFNHIKDSGYYATNEEIEIFLESCTLCKEITAQTKRNSYKKRNI INKLPEEEEEEEEEEEEEEEEEEQEEEVEKPTISEEEEEETPAVSEEEKEEEEQEEDK EKDKEKKIEEDTETGKKKDEVMNERQDGIEISEKTNDKPTEKTKVKKNVSRVKDINLH KKGNPITPKKIKNFSQQQLRLASGVSNEKRAIITLKK" rep_origin 2869..3411 /note="Ddp1 ori" rep_origin 3686..4353 /note="pUC ori" CDS complement(4501..5361) /codon_start=1 /transl_table=11 /product="penicillin beta-lactamase" /protein_id="ACH81564.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory complement(5513..5768) /regulatory_class="terminator" /note="mhcA terminator" CDS complement(5775..6845) /codon_start=1 /transl_table=11 /product="hygromycin B phosphotransferase" /protein_id="ACH81565.1" /translation="MDQFNISQQDLMKKPELTATSVEKFLIEKFDSVSDLMQLSEGEE SRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCI SRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRD FICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTD NGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAY MLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVE VLADSGNRRPSTRPDREMGEAN" regulatory complement(6857..7078) /regulatory_class="promoter" /note="act6 promoter" BASE COUNT 2290 a 1243 c 1154 g 2392 t ORIGIN 1 ctcgagacta gagctagata aaaaaaattt ttatttattt ttatttattt tgaattaaat 61 agattacaaa ttaattaatc ccatcaaatc tttaaaaaaa aatggtttaa aaaaacttgg 121 gttggttaat tattatttga aaattttaaa acccaaatta aaaaaaaaaa atgggattca 181 aaaatttttt tttttttttt tttttttttt tttttttttt tttcagattg cataaaaaga 241 tttttttttt ttttttttct tatttcttaa aacaaataaa ttaaattaaa taaaaaataa 301 aaatcagatc tagtactagt taaataaata aattatttaa taaataataa aaaaacaaat 361 tgttgtaata atctaatatt ttcttttttt tttaattttt tttttttaaa tcttaataat 421 tattaagtta ttttaatttt tttttttttt tttttttttt tttttttttt ttttctatca 481 aaaaaatcaa atatatttaa aaaatttatt atttacagat acattttgaa tggtgaagat 541 aaatatatgc attagatgta aaacagccaa agagtatgaa aatcaaaaag ataaagctta 601 tcgatttcga aaaagtaaat agcaattatt acaaaattca atccgaatct acccaaataa 661 attccaatga aattgccgat ttaaaaaagt ttattaaaga agaagtcaat aaaacttctt 721 ccaaaattga tttcttttta gtttcttcaa cagatgccct ttcaaatcca gaaaattatt 781 ctctcttaga agtaaagtgt attaattgtc attctttgtg tcaaggaaaa aatttatata 841 tttcatgtac aagagatgga tgtcaaaaca atatttgcta taattgttta ggaataaaca 901 taaacatata taatgttgtt attaattcta aactttgccc tccatgtttc aatgattcgg 961 taatcaacaa gaagtgtgcc atgtgtagta agaacggaac taaatgtaat ttgaaccaag 1021 aatgtaaact tcatctttgt gcacagtgtt ctaaaaagtg tctatacatt ctgagagtca 1081 aaactaatta aataaaatat aaacttaatt tctaaataaa ctcatttaaa aatatttaaa 1141 taatatgaat ttataactgt aattattgta ttaaaaaatt atataattat ttaatgttaa 1201 aaatgtatta aaataattat aaaaaaatat aacaaaaatt ttcgtaaaaa taatttgtaa 1261 aaaagctatt aaaaatatta tgaaaaaaaa attaaaaaaa ttattaaatt gtttttgtaa 1321 ttaagctatt aaaataatta taaaaaaaaa atttttaaaa ttttaaaaat attttttgta 1381 aaaaagtatt aaaataatta tgaaaaaaaa attttctaaa aaattaaaaa aaaaattaaa 1441 atatatttta tgttaaaaac gtattaaaat aactattaaa aaaattatat ttaaaaaagt 1501 attaactttt ttttaggtgt ggttgtgggg tggggtttaa tatattataa taaaaaatta 1561 ttttttgttc atttattatt ttcattgtat ataatgtact caacaacgtt attatttttt 1621 cttttttttt ttattgtatc aaaatcttct gttcttcaaa atgatcagat tgaagtaaaa 1681 tattttcaac ttcttattgt tatgtatcaa aaagaaaact gtgttgaaaa gtcaatgaca 1741 ggcgccgtaa tttatgatga atgtaatatt catggaagag ttgaaacaaa tagtactcat 1801 gcgctttttt atgatgacat tgaaacaaat aattcaagat gtaacaattt tcgtaattta 1861 acaaacttaa ttaaacttaa tgaatgtatt aatgacgagt ttggagagtc tattctttat 1921 aaagaatata atgaaactga tgatggttat ttgtttagag tggaagacag ctttgttgaa 1981 attacttctc tttcaatgga ttgtacaaaa aatagtaaaa caattattga aaaattcaac 2041 atttgttcaa aatttgaaaa tgtatatcat attacaaaca ttacacaaga gaaatccaat 2101 agatttacat gtacagatcc attgtgccac tattgtaaga atgaaaacat tcaaaacaat 2161 cttgatttta aaacaacaaa gtgtactcca aagtatggtg catctgattc tgaattttta 2221 tcaacaattt acaatccaaa gctcgatggc tcaaataacg gtatggaaaa gtcagtaact 2281 caagaaaaaa acatttcaaa taatttaaaa attaatatat atttaatttt ctttttaatt 2341 atttttttaa ttaaataaag ttttattatt ttttaagagt aattattgct cttttttcat 2401 ttgaaacacc agaagctaaa cgtaattgtt gttgactgaa attttttatt ttttttgggg 2461 taataggatt tcctttttta tgaagattaa tatctttgac tcgtgaaaca ttctttttaa 2521 cttttgtttt ttctgttggt ttatcatttg ttttttcact aatttcaata ccatcttgac 2581 gttcattcat aacttcatct tttttttttc ctgtttctgt atcttcttct attttttttt 2641 ctttatcttt ttctttatct tcttcttgtt cttcctcttc tttttcttct tctgatactg 2701 caggtgtttc ttcttcttct tcttccgata ttgtcggttt ttctacttct tcttcttgtt 2761 cttcttcttc ttcttcttct tcttcttctt cttcttcttc ttcttcttct tccggtaatt 2821 tattaattat atttcttttt ttatatgaat tacgtttggt ttgtgcagta atttccttac 2881 atagagtgca gctttcaaga aaaatttcaa tttcttcgtt tgttgcataa taaccactgt 2941 ctttgatatg attaaacatt tttgattttc ttaaatgctt tccttcttta atatgaaaat 3001 tatcgaattc taattcatta agaacaataa gctcccctaa tttaaaaaat tagttaaaat 3061 aaattaaaat gaacatgtat aaagatggat tttaccattt tttgaaattc taaataactt 3121 ttcttcatct ccaatctttt tgactgaaaa acgattttta attgaagtta ttgttctgtg 3181 agtgttttga atcgcccatt tctctaaatc agtttgagat agtgttttat aatctgaatt 3241 gttatacaca acttttgctc tattaaccaa atatttaaag atttcatcat caactgaata 3301 ttttgacttt acgattcttg tccaaaaaac aatttctact actatcattt tttatttata 3361 aaataattta aatacaaaaa tgaatttttt tttttttaaa aaaaaaaaaa tttgaaaaaa 3421 aaaaaaaaaa aattttaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa tcaaataaaa 3481 agtaaaaaat aaaaaccgaa aaacattcat tgtaatttca aatgtcgagg ccggcagagg 3541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 3601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 3661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 3721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 3781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 3841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 3901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 3961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 4021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 4081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 4141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 4201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 4261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 4321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 4381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 4441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 4501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 4561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 4621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 4681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 4741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 4801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 4861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 4921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 4981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 5041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 5101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 5161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 5221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 5281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 5341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 5401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 5461 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggga tccattttat 5521 ttaatatact aaataataaa aaagttaaaa aatgatcatt ggataaattt tttataatta 5581 taaataaaga taataatttt ttttttaaca aaactaaaaa taaaaataat aaaataattg 5641 ttaaaatagg tttttttttt tttttttttt ttttaataaa tggtatttat taatttattt 5701 gttgtgtgtg tttttttttt tataatattt ttttttttag cattgaatta agaagaaatc 5761 aaattgattc tagtttagtt agcctccccc atctcccgat ccggacgagt gctggggcgt 5821 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg 5881 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga 5941 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca 6001 agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc 6061 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg 6121 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt 6181 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg 6241 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca 6301 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat 6361 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg 6421 ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga 6481 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag 6541 atgcaatagg tcaggctctc gctaaattcc ccaatgtcaa gcacttccgg aatcgggagc 6601 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta 6661 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg 6721 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg 6781 acagacgtcg cggtgagttc aggctttttc attaggtctt gttgagaaat gttaaattga 6841 tccattttgg atcttttata ttatatttat ttattgatta tttttttgaa ttaattaaaa 6901 aaaaaaaaaa tttcatttta taatctcaga aacctcaaaa aaaaaaaaat aaaaaataaa 6961 aaatataaaa aaataaaaat aaaatcccaa ttttaaagcg aaaaaccacc catggtttga 7021 aaatttcaat caatttcaaa taactttact taaaaaaaac ccatttttta tttaaaaaa //