LOCUS EL608842 690 bp mRNA linear EST 07-MAR-2007
DEFINITION EoEST0856 Oil palm mesocarp-tissue cDNA Entry Library Elaeis
oleifera cDNA clone Mo17-838 5' similar to Unknown protein [Note,
similar to Oryza sativa (japonica cultivar-group) clone
Os12g0238100, genbank accession No: AP008218], mRNA sequence.
ACCESSION EL608842
VERSION EL608842.1
DBLINK BioSample: SAMN00152624
KEYWORDS EST.
SOURCE Elaeis oleifera (American oil palm)
ORGANISM Elaeis oleifera
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; Liliopsida; Arecaceae; Arecoideae;
Cocoseae; Elaeidinae; Elaeis.
REFERENCE 1 (bases 1 to 690)
AUTHORS Bhore,S.J., Amelia,K., Nurhuda,J. and Shah,F.H.
TITLE Analysis of expressed sequence tags (ESTs) generated from Elaeis
oleifera mesocarp tissue cDNA library (2007b)
JOURNAL Unpublished
COMMENT Contact: Bhore S.J.
Molecular Biology
Melaka Institute of Biotechnology
Lot 7, MITC City, Ayer Keroh, 75450, Melaka, Malaysia
Tel: 006 6 231 3532
Fax: 006 6 231 3276
Email: subhashbhore@yahoo.com
Insert length is not determined by restriction enzyme (BsrGI)
analysis. Only kanamycin resistant E. coli were selected,
cultivated, and ESTs were generated. Elaeis oleifera, seventeen
weeks old [Weeks After Anthesis (WAA)] mesocarp tissue cDNA library
was constructed using the CloneMiner cDNA library construction kit.
The cDNA carrying entry clones (pDNAs) were used in transformation
of E. coli and kanamycin resistant E. coli were selected,
cultivated, and ESTs were generated. After trimming and processing,
analysis of nucleotide sequences of ESTs has been completed, and
submitted in dbEST.
Seq primer: Universal primer M13 (Forward); 5' GTAAAACGACGGCCAG 3'
POLYA=No.
FEATURES Location/Qualifiers
source 1..690
/organism="Elaeis oleifera"
/mol_type="mRNA"
/cultivar="oleifera (Malaysian)"
/db_xref="taxon:80265"
/clone="Mo17-838"
/cell_type="Somatic"
/tissue_type="Mesocarp"
/clone_lib="SAMN00152624 Oil palm mesocarp-tissue cDNA
Entry Library"
/dev_stage="17 week after anthesis (WAA)"
/note="Organ: Fruit; Vector: Donor Vector, pDONR 222;
Site_1: BsrGI; Site_2: BsrGI; The cDNA library was
prepared following all the guidelines and the protocols
provided by manufacturer (Invitrogen Corporation) of the
CloneMiner cDNA Library Construction Kit. This CloneMiner
cDNA Library Construction Kit was used because it enables
us to construct highly representative cDNA libraries
without the risk of restriction enzymes cutting our
precious cDNA clones. Therefore, we can isolate fully
intact clones from high-quality cDNA library."
BASE COUNT 181 a 139 c 186 g 184 t
ORIGIN
1 tgctgtggtc gtcgcctccg atgtttccgg cggtggatga actcctcgct gtgttcaagg
61 attcctgcaa ggagttggtt gatctccgac gccagattga cgcaaggctc caaaatctaa
121 agaaagatgt tgccatccaa gatgctaagc accgcaaaac cctgaccgag ctggaaaaag
181 gtgtagatgg cttatttgat agctttgcaa gactggatac ccggattgct agtgtgggtc
241 agactgctgc gaagattggg gatcatttgc agagtgcaga ttctcagaga gaaactgcca
301 atcaaacaat agagcttatt aagtacttga tggaattcaa cagcagccct ggtgatctta
361 tggaactttc acctctgttt tctgatgaca gtcgtgttgc tgaggctgct tcaattgcac
421 agaaattgcg ggcttttgct gaggaggatg ttggaaaaca gggcatgtca tccgcagtgg
481 gtgcggcggc tccaagcagg ggtcttgaag ttgcagctgc aaatcttcaa gactattgca
541 atgagttgga aaatagattg ttggctcggt ttgatgcagc atcccagaag cgaagaatta
601 tctattaatg gcagaatgtg ctaaaatttg tctcagttta acaggggtac cagtgcctgg
661 caacattatg tagcatcacg accaatgttt
//